The anti-estrogenic activity of selected polynuclear aromatic hydrocarbons in yeast expressing human estrogen receptor

Biochemical and Biophysical Research Communications
D Q TranS F Arnold

Abstract

Polynuclear aromatic hydrocarbons (PAH) represent a large class of chemicals present in environment. We have used yeast strain ER(wt) expressing human estrogen receptor (hER) and an estrogen-sensitive reporter to characterize the estrogenic or anti-estrogenic activities of 21 PAHs. The PAHs did not exhibit estrogenic activity in yeast strain ER(wt). Four of the PAHs, dibenz[a,h]anthracene, 6-hydroxy-chrysene, 2,3-benzofluorene, and benzo(a)pyrene, inhibited estradiol-dependent reporter activity in strain ER(wt). A mutant hER lacking the amino terminus expressed in yeast was inhibited by the four PAHs to a lesser extent than the full-length hER. 6-hydroxy-chrysene, 2,3-benzofluorene, and benzo(a)pyrene, but not dibenz[a,h]anthracene, weakly displaced [3H]estradiol from the hER in a competition binding assay. A strong correlation between the inhibition of [3H]estradiol-binding from the hER and the reduction of hER-mediated transactivation in yeast was not observed. These observations suggest that the PAHs dibenz[a,h]-anthracene, 6-hydroxy-chrysene, 2,3-benzofluorene, and benzo(a)pyrene may antagonize activity in yeast by binding to an anti-estrogen binding site on the hER or by mechanisms independent of the hER.

Citations

Jun 19, 2004·Journal of Toxicology and Environmental Health. Part a·John SsempebwaKathleen Arcaro
Feb 3, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Naoya KishikawaNaotaka Kuroda
May 20, 2008·Expert Opinion on Drug Metabolism & Toxicology·José M Navas, Helmut Segner
Apr 17, 2012·Ecotoxicology and Environmental Safety·Fei LiWillie J G M Peijnenburg
Mar 1, 2012·Aquatic Toxicology·Karina Petersen, Knut Erik Tollefsen
May 4, 2010·Ecotoxicology and Environmental Safety·Maren Ortiz-Zarragoitia, Miren P Cajaraville
Jun 16, 2015·Environment International·Ryoiti Kiyama, Yuko Wada-Kiyama
Jun 3, 2008·Journal of Hazardous Materials·John C Ssempebwa, David O Carpenter
Dec 14, 2004·Toxicology and Applied Pharmacology·Grantley D CharlesM Sue Marty
Sep 1, 2014·Environmental Research·Sandie HaXiaohui Xu
Mar 3, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Meng XiuJingjing Miao
Sep 30, 1999·Toxicology and Applied Pharmacology·E OberdörsterJ A McLachlan
Apr 24, 2014·Journal of Toxicology and Environmental Health. Part a·P GeraudieL Camus
May 3, 2005·The Science of the Total Environment·Lesley J Mills, Clinton Chichester
Sep 3, 2004·Environmental Science and Pollution Research International·Andrew C RastallLothar Erdinger
Jan 1, 2000·Environmental Science and Pollution Research International·K HilscherovaJ P Giesy
Apr 30, 2008·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Hueiwang Anna Jeng, Liang Yu
Aug 30, 2017·Journal of Toxicology and Environmental Health. Part a·Maria T HultmanKnut Erik Tollefsen
May 13, 2005·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Ndiya OgbaSamir Raychoudhury
Jun 19, 2001·Journal of Applied Toxicology : JAT·P SohoniJ P Sumpter
Mar 10, 2015·Reproduction, Fertility, and Development·Michał RadwanWojciech Hanke
Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M JørgensenH Leffers
Aug 2, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I BoonenH Demaegdt
Oct 23, 2008·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Ryan L WilliamsRobert I Krieger
Nov 26, 2019·Toxicology Reports·Joycelyn SmithSamir Raychoudhury
May 13, 2015·General and Comparative Endocrinology·Christopher J Kennedy, Kevin R Smyth
May 19, 2009·Analytical Chemistry·Hua-Ling XuXiu-Ping Yan
Apr 29, 2021·Environmental Science & Technology·Gabrielle P BlackThomas M Young
Mar 18, 2000·Toxicology Letters·C M Lye

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