The Anti-inflammatory Effect of GV1001 Mediated by the Downregulation of ENO1-induced Pro-inflammatory Cytokine Production

Immune Network
Jiyea ChoiWang Jae Lee

Abstract

GV1001 is a peptide derived from the human telomerase reverse transcriptase (hTERT) sequence that is reported to have anti-cancer and anti-inflammatory effects. Enolase1 (ENO1) is a glycolytic enzyme, and stimulation of this enzyme induces high levels of pro-inflammatory cytokines from concanavalin A (Con A)-activated peripheral blood mononuclear cells (PBMCs) and ENO1-expressing monocytes in healthy subjects, as well as from macrophages in rheumatoid arthritis (RA) patients. Therefore, this study investigated whether GV1001 downregulates ENO1-induced pro-inflammatory cytokines as an anti-inflammatory peptide. The results showed that GV1001 does not affect the expression of ENO1 in either Con A-activated PBMCs or RA PBMCs. However, ENO1 stimulation increased the production of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, and these cytokines were downregulated by pretreatment with GV1001. Moreover, p38 mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB were activated when ENO1, on the surface of Con A-activated PBMCs and RA PBMCs, was stimulated, and they were successfully suppressed by pre-treatment with GV1001. These results suggest that GV1001 may be an effect...Continue Reading

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Citations

Apr 29, 2016·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Ji-Eun ChangKwhanmien Kim
Oct 18, 2016·Journal of Ginseng Research·Kwang-Soo BaekJae Youl Cho
Apr 4, 2017·The American Journal of Chinese Medicine·Jae Sik YuJae Youl Cho
Feb 7, 2018·Frontiers in Cellular Neuroscience·Shin Hye KimJa-Won Koo
Jul 18, 2018·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·So Young KimJa-Won Koo
Sep 26, 2017·Molecular Medicine Reports·Ji-Eun ChangCheong Lim
Apr 25, 2019·International Journal of Molecular Sciences·Eishiro Mizukoshi, Shuichi Kaneko
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yixian ZhangChangyou Gao
Sep 24, 2019·Current Aging Science·Joan Smith-Sonneborn
Jul 18, 2018·International Journal of Molecular Medicine·Kyeong Seok KimHyung Sik Kim

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Methods Mentioned

BETA
flow cytometry
FACS
ELISA

Software Mentioned

SoftmaxPro
ImageJ
GraphPad In - Stat
FlowJo
GraphPad

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