The Anti-inflammatory Effect of Hydrogen on Lung Transplantation Model of Pulmonary Microvascular Endothelial Cells During Cold Storage Period

Transplantation
Guangchao ZhangHuacheng Zhou

Abstract

Lung ischemia-reperfusion injury (LIRI) remains an important factor for the early mortality of lung transplantations. Hydrogen (H2) can attenuate lung injury and improve lung function in animal experiments. In previous studies, pulmonary microvascular endothelial cells (PMVECs) were used to simulate LIRI. We hypothesized that H2 can alleviate inflammatory injury in a PMVECs lung transplantation model in the cold ischemia phase. Pulmonary microvascular endothelial cells were divided into 4 groups: blank, control, oxygen (O2), and H2. The blank group included PMVECs without treatment. During the cold storage period, the O2 group was aerated with 40% O2 and 60% N2, and the H2 group was aerated with 3% H2, 40% O2, and 57% N2. The control group was aerated without gases. The mixed gases were replaced every 20 minutes for 4 hours. During the transplantation period, the sealed containers were warmed for 1 hour at room temperature. In the reperfusion period, the containers were aerated with 50% O2, 5% CO2 and 45% N2 at 37°C. The concentrations of interleukin-6 and tumor necrosis factor-α in the extracellular solutions were significantly decreased, and the concentration of interleukin-10 was increased in the H2 group. Intercellular adhe...Continue Reading

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Citations

Aug 31, 2019·General Thoracic and Cardiovascular Surgery·Naoki OzekiAkihiko Usui
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Jul 11, 2020·The Annals of Thoracic Surgery·Hidenao KayawakeHiroshi Date
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Apr 27, 2021·Experimental Biology and Medicine·Lini QuanHuacheng Zhou
Aug 9, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qiongge HuAnwen Shao

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