The anti-inflammatory interactions of epinephrine with human neutrophils in vitro are achieved by cyclic AMP-mediated accelerated resequestration of cytosolic calcium

Biochemical Pharmacology
G TintingerJ A Ker

Abstract

This study was designed to evaluate the effects of epinephrine (0.01-1 microM) on superoxide production by, and release of elastase from human neutrophils activated with the chemotactic tripeptide, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) (1 microM) in vitro, and to relate alterations in these responses to changes in adenosine 3,5' cyclic monophosphate (cAMP) and cytosolic free Ca(2+). Cyclic AMP, superoxide production and elastase release were measured by radioimmunoassay, lucigenin-enhanced chemiluminescence, and a colorimetric procedure respectively. Cytosolic Ca(2+) fluxes were measured by fura-2 spectrofluorimetry in combination with radiometric procedures that enable distinction between net efflux and influx of the cation. Epinephrine treatment of neutrophils resulted in increased cAMP and dose-related inhibition of both superoxide production and elastase release, which was potentiated by the type 4 phosphodiesterase inhibitor, rolipram, and attenuated by propranolol, but not by selective beta(1)-, alpha(1)- or alpha(2)-adrenoreceptor antagonists. Although epinephrine did not affect the FMLP-activated abruptly-occurring increase in fura-2 fluorescence intensity, indicating no effects on the release of Ca(2+) f...Continue Reading

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Citations

Nov 2, 2004·European Journal of Applied Physiology·E OrtegaA B Rodríguez
Aug 2, 2007·European Journal of Applied Physiology·Tzai-Li Li, Pei-Yun Cheng
Sep 27, 2003·Lancet·Gustavo Daher Vieira de Moraes Barros, Carla Regina de Carvalho Leite
Feb 14, 2002·Free Radical Biology & Medicine·Maria Cristina PolidoriPatrizia Mecocci
Sep 4, 2003·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Leslie M GimenezMark E Nordness
Dec 3, 2002·Current Controlled Trials in Cardiovascular Medicine·Linda B PillerUNKNOWN The ALLHAT Collaborative Research Group
Nov 27, 2008·Journal of Negative Results in Biomedicine·Izabela Grabska-KobyleckaDariusz Nowak
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Sep 15, 2004·European Journal of Pharmacology·Alan SabirshChrister Owman
Aug 31, 2004·Journal of Applied Physiology·Alexander ChoukèrManfred Thiel
Apr 30, 2009·Pediatrics International : Official Journal of the Japan Pediatric Society·Fumiko YamatoKazunari Kaneko
Sep 29, 2007·Journal of Applied Physiology·Stewart J LaingNeil P Walsh
Dec 14, 2004·Biochemical Pharmacology·Tsong-Long HwangReen-Yen Kuo

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