The Anti-Müllerian Hormone Precursor (proAMH) Is Not Converted to the Receptor-Competent Form (AMHN,C) in the Circulating Blood of Mice

Endocrinology
Michael W PankhurstIan S McLennan

Abstract

Anti-Müllerian hormone (AMH) is a gonadal hormone that regulates aspects of male sexual differentiation and ovarian function. AMH is synthesized as the AMH proprotein precursor (proAMH), which is converted to a receptor-binding form (AMHN,C) by proteolytic cleavage. ProAMH appears to be the predominant species in the ovary, whereas AMHN,C is the prevalent form in circulation. The aim of this study was to determine whether cleavage of proAMH occurs before it is released from the gonad or while in circulation. The individual half-lives of the proAMH and AMHN,C were also determined, as this has important implications for understanding the mechanisms of AMH signaling. Recombinant human (rh)-proAMH or rh-AMHN,C was injected iv into mice. AMH levels were analyzed in a series of repeated blood samples using an assay that detects human, but not murine, AMH. The degree of cleavage of injected proAMH was assessed by immunoprecipitation and Western blotting. The elimination half-life curves were biphasic. The fast-phase elimination was estimated at 6 and 11 minutes for rh-proAMH and rh-AMHN,C, respectively. The slow-phase half-life estimates were 2.4 and 3.8 hours for rh-proAMH and rh-AMHN,C, respectively. Immunoprecipitation of rh-proAMH...Continue Reading

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Citations

Apr 1, 2016·Scientific Reports·Ghislaine GarrelJoëlle Cohen-Tannoudji
Mar 16, 2018·The Journal of Endocrinology·Michael W PankhurstNicola J Batchelor
Mar 13, 2018·The Journal of Veterinary Medical Science·Anzu YamamotoNobuhiko Hoshi
Oct 16, 2018·Current Opinion in Endocrinology, Diabetes, and Obesity·David PépinPatricia K Donahoe
Jul 2, 2019·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A Ya RakA M Ischenko
Nov 28, 2017·Biology of Reproduction·Ian S McLennanMichael W Pankhurst
Apr 15, 2021·Endocrine Reviews·Nathalie di ClementeJoëlle Taieb
Oct 9, 2021·Biomedical Chromatography : BMC·Alexandra Ya RakAlexander M Ischenko

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