The anti-tumor role and mechanism of integrated and truncated PDCD5 proteins in osteosarcoma cells

Cellular Signalling
Xiao-Rui HanXi-Zhuang Bai

Abstract

Osteosarcoma (OS) is a high-grade malignant bone tumor. In these studies, the cell apoptosis-related gene, programmed cell death 5 gene (PDCD5), and various fragments of it, were overexpressed in the OS cell line, MG-63. The effects of PDCD5 on MG-63 cells both in vivo and in vitro were then identified. Our results indicate that PDCD5 can induce apoptosis and G(2) phase arrest in MG-63 cells. Moreover, expression of PDCD5 in established xenografted tumors was associated with a decrease in tumor size and weight. Accordingly, the survival rate of these mice was significantly higher than that of mice bearing tumors that did not express PDCD5. To analyze the signaling pathway involved, western blotting was performed. In these assays, PDCD5 was found to inhibit the Ras/Raf/MEK/ERK signaling pathway, leading to inhibition of cyclin B and CDK1. In addition, down-regulation of ERK resulted in activation of caspase 3 and caspase 9. These results are consistent with the G(2) phase arrest observed with overexpression of PDCD5. However, a G(1) phase arrest was not observed. Therefore, proteins associated with the G(1) phase of the cell cycle were overexpressed in combination with PDCD5 overexpression. Overall, these studies demonstrate the...Continue Reading

References

Apr 1, 1997·The Journal of Clinical Investigation·V S SivaramanC C Malbon
Sep 1, 1997·Molecular and Cellular Biology·A SewingH Land
Jul 29, 1999·The New England Journal of Medicine·C A Arndt, W M Crist
Jul 24, 2003·The Journal of Cell Biology·Jill K Slack-DavisAndrew D Catling
May 4, 2004·Biochemical and Biophysical Research Communications·Dongsheng LiuJinfeng Wang
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·I ZucchiR Dulbecco
Jan 6, 2005·Journal of Biochemistry·Satoru ToriiEisuke Nishida
Dec 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·L N ChenY Y Chen
Feb 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·S TuveB M Pützer
Mar 21, 2006·Apoptosis : an International Journal on Programmed Cell Death·Y-H YangY-Y Lu
Mar 22, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Monica SpinolaAndreas Braun
Apr 11, 2008·Apoptosis : an International Journal on Programmed Cell Death·Guo-Rui RuanXiao-Jun Huang
Aug 14, 2008·Expert Review of Anticancer Therapy·Herrick J Siegel, Joseph G Pressey
Nov 12, 2009·Nature Reviews. Molecular Cell Biology·Patrick T CaswellJim C Norman
Mar 30, 2010·Apoptosis : an International Journal on Programmed Cell Death·Changbao ChenYingyu Chen

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Citations

Jun 4, 2013·European Journal of Pharmacology·Yedong ChengYang Yang
Nov 14, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lanlan WangLu Wang
Nov 26, 2015·Journal of Microencapsulation·Damla Çetin Altındal, Menemşe Gümüşderelioğlu
Jan 19, 2016·Biochimica Et Biophysica Acta·Ge LiYingyu Chen
Jan 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wei-Wei TongYong Liu
Oct 15, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Yu ZhangYingren Zhao
Sep 5, 2015·Journal of Orthopaedic Surgery and Research·Xiao-Rong YangRen-Rong Gong
Nov 22, 2015·Cellular Oncology (Dordrecht)·Wei WangCheng-Hai Zhao
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dan WangPu Xia
Jun 13, 2015·Journal of Neuroinflammation·Juan XiaoWenbin Deng
Jun 29, 2013·International Journal of Oncology·Da-Zhi FuYong-Feng Liu
Feb 7, 2018·Oncology Letters·Penghui LiLihong Zheng
Nov 9, 2016·International Journal of Oncology·Wei WangCheng-Hai Zhao
Dec 5, 2019·Cancer Cell International·Hadis FathizadehZatollah Asemi

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