The antiangiogenic activities of ethanolic crude extracts of four Salvia species

BMC Complementary and Alternative Medicine
Malek ZihlifRanda Naffa

Abstract

Angiogenesis is one of cancer hallmarks that are required for both cancer progression and metastasis. In this study we examined the antiangiogenic properties of the ethanolic crude extracts of four Salvia species grown in Jordan. The direct antiangiogenic activity was evaluated using various models: ex vivo rat aortic ring assay, in vitro assessment of HUVEC proliferation and migration, and in vivo CAM assay, while we used the changes in the expression of HIF-1α and VEGF in breast cancer cells (MCF 7) as an indicative for the indirect antiangiogenic activity. All four crude extracts showed a potential antiangiogenic activity in the rat aortic assay, however two species were found to be cytotoxic against Fibroblast cell line (PLF); the finding that caused the exclusion of these two extracts from further studies. Of the two remaining extracts, S. triloba showed very promising direct and indirect antiangiogenic activities. S. triloba inhibited the HUVEC proliferation with an IC50 of 90 μg/mL and HUVEC migration by 82% at 150 μg/mL. Furthermore, the in vivo CAM assay also illustrated the high impact of S. triloba against the newly formed vessel in the chicken embryonic membrane. Interestingly, the S. triloba inhibited the expressio...Continue Reading

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Citations

Sep 25, 2014·BMC Complementary and Alternative Medicine·Jin-Mu YiNo Soo Kim
Feb 4, 2016·Journal of Economic Entomology·Jelena BarbirJosé Dorado
Jul 22, 2014·Natural Product Research·Monica Rosa LoizzoRosa Tundis
Sep 15, 2016·Expert Review of Hematology·E Carlos Rodriguez-Merchan
Jan 20, 2017·Nutrition and Cancer·Gilead Ebiegberi ForcadosPeace Mabeta
Feb 18, 2018·BMC Complementary and Alternative Medicine·Malek ZihlifRanda Naffa
Jun 15, 2018·Molecular Medicine Reports·Qasem AbdallahAmin Abdulmajid

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Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

Leica Quin
GraphPad Prism
SSPS

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