The antiapoptotic OPA1/Parl couple participates in mitochondrial adaptation to heat shock.

Biochimica Et Biophysica Acta
Luiza K Sanjuán Szklarz, Luca Scorrano

Abstract

The mitochondria-shaping protein optic atrophy 1 (OPA1) has genetically distinguishable roles in mitochondrial morphology and apoptosis. The latter depends on the presenilin associated rhomboid like (PARL) protease, essential for the accumulation of a soluble intermembrane space form of OPA1 (IMS-OPA1). Here we show that OPA1 and PARL participate in the heat shock response, a stereotypical cellular process of adaptation to thermal stress. Upon heat shock, long forms of OPA1 are lost and mitochondria fragment. However, mitochondrial fusion is dispensable to maintain viability, whereas IMS-OPA1 is required. Upon conditioning-a process of mild heat shock and recovery-IMS-OPA1 accumulates, OPA1 oligomers increase and mitochondria release less cytochrome c, ultimately resulting in cellular resistance to subsequent apoptotic inducers. In Parl(-/-) cells accumulation of IMS-OPA1 is blunted and conditioning fails to protect from cytochrome c release and apoptosis. Thus, the OPA1/PARL dependent pathway of cristae remodeling is implicated in heat shock. This article is part of a Special Issue entitled: 17th European Bioenergetics Conference (EBEC 2012).

References

Feb 15, 1994·Microscopy Research and Technique·J Bereiter-Hahn, M Vöth
Aug 22, 2001·Molecular Biology of the Cell·E SmirnovaA M van der Bliek
Jan 10, 2002·Developmental Cell·Luca ScorranoStanley J Korsmeyer
Nov 20, 2002·Circulation·S Gupta, A A Knowlton
May 22, 2003·Journal of Cell Science·Ansgar SantelMargaret T Fuller
May 30, 2003·Nature·G Angus McQuibbanMatthew Freeman
Jun 5, 2003·The Journal of Biological Chemistry·Dominic I JamesJean-Claude Martinou
Aug 25, 2004·Science·Michael S Wolfe, Raphael Kopan
Sep 10, 2004·Molecular Biology of the Cell·Yang-ja LeeRichard J Youle
Oct 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sara CipolatLuca Scorrano
May 19, 2005·The Journal of Biological Chemistry·Hsiuchen ChenDavid C Chan
Jun 17, 2006·The EMBO Journal·Naotada IshiharaKatsuyoshi Mihara
Sep 28, 2006·The Journal of Biological Chemistry·Stéphane Duvezin-CaubetAndreas S Reichert
Apr 28, 2007·Cell Death and Differentiation·L Pellegrini, L Scorrano
Jul 7, 2007·Molecular Biology of the Cell·Stéphane Duvezin-CaubetAndreas S Reichert
Aug 22, 2007·The Journal of Cell Biology·Lorena GriparicAlexander M van der Bliek
Jan 25, 2008·The EMBO Journal·Takashi Tatsuta, Thomas Langer
Apr 19, 2008·Journal of Neurochemistry·Vasudheva Reddy AkepatiChristiane Alexander
Apr 11, 2009·The EMBO Journal·Daniel TonderaJean-Claude Martinou
May 5, 2009·Experimental Cell Research·Kristin MöpertAnsgar Santel
Aug 4, 2009·Trends in Endocrinology and Metabolism : TEM·Michał Wasilewski, Luca Scorrano
Sep 16, 2009·The Journal of Cell Biology·Rachel M DeVayJodi Nunnari
Dec 30, 2009·The Journal of Cell Biology·Sarah EhsesThomas Langer
Oct 23, 2010·Molecular Cell·Klaus RichterJohannes Buchner
Dec 1, 2010·The Journal of Cell Biology·Seok Min JinRichard J Youle
Apr 5, 2011·Cell·Maria Eugenia Soriano, Luca Scorrano

❮ Previous
Next ❯

Citations

Nov 5, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·R CanzoneriM C Abba
Jul 23, 2013·European Journal of Clinical Investigation·Luca Scorrano
Aug 14, 2012·Biochimica Et Biophysica Acta·Mafalda Escobar-Henriques, Fabian Anton
Aug 19, 2014·Journal of Molecular Neuroscience : MN·Kasthuri Bai MagalingamNagaraja Haleagrahara
Sep 23, 2014·Science·Douglas R GreenGuido Kroemer
Oct 27, 2016·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·M C RusuN Mirancea
Aug 6, 2016·PloS One·Haitham E El-SikhryJohn M Seubert
Aug 25, 2017·Scientific Reports·Valentina Di PietroAntonio Belli
Oct 9, 2013·Biochimica Et Biophysica Acta·Eliana Y L Chan, G Angus McQuibban
Sep 5, 2021·Science China. Life Sciences·Deyang YaoXingguo Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
two hybrid
flow cytometry
FACS
confocal microscopy
thermal stress

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.