The antibody-enzyme analogy. Comparison of enzymes and antibodies specific for phosphopyridoxyltyrosine

V Raso, B D Stollar


Reduced Schiff base compounds of pyridoxal-P and tyrosine, which were used to induce specific antibodies described in the preceding article (V. Raso and B. D. Stolar, Biochemistry, 1975), caused active site-directed inhibition of tyrosine transaminase and tyrosine decarboxylase. The antibodies, studied as analogs of enzymes, were able to bind an unsaturated Schiff base catalytic intermediate, as shown by equilibrium dialysis and absorbance difference spectroscopy. Schiff base formation can proceed while the pyridoxal-P and tyrosine are within the antibody combining site, but the rate of this bimolecular condensation within the sites was not greater than the rate in free solution. Antibody did effect a small rate enhancement for the pyridoxal-P-catalyzed transamination of L-tyrosine. These results are discussed in light of current ideas in the mechanisms of enzyme catalysis.


Feb 1, 1991·Journal of Protein Chemistry·G Rao, M Philipp
Aug 15, 1984·Experientia·P DominiciC Borri Voltattorni
Apr 24, 1999·Applied Biochemistry and Biotechnology·S Paul
May 1, 1994·Applied Biochemistry and Biotechnology·N R Thomas
Feb 1, 1994·Molecular Biotechnology·D S TawfikB Green
Jan 6, 2005·Springer Seminars in Immunopathology·Sudhir PaulMarc E Weksler
Oct 16, 2002·Journal of Immunological Methods·S I GramatikovaP Christen
Mar 6, 1999·Molecular Immunology·E LiuJ S Lee
Jun 20, 1997·Biochimica Et Biophysica Acta·U B HanssonU Alkner
Mar 1, 2002·The New England Journal of Medicine·B D Stollar
Jan 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Natalia PonomarenkoAlexander Gabibov
Dec 1, 1996·Natural Product Reports·N R Thomas
Oct 2, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexey BelogurovAlexander Gabibov
Oct 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R A Lerner, S J Benkovic
Nov 19, 2005·Research in Microbiology·Jean-Luc Jestin, Sophie Vichier-Guerre
Dec 13, 2005·Immunology Letters·Alexander Gabibov
Jun 1, 2001·Methods : a Companion to Methods in Enzymology·K BrocklehurstC M Topham
Jan 1, 1993·International Reviews of Immunology·J D Stewart, S J Benkovic
Nov 27, 1998·Nature Biotechnology·M C FletcherJ S Lee
Jul 5, 2017·Infection and Immunity·Anthony BowenArturo Casadevall
Nov 29, 1996·The Journal of Biological Chemistry·S I Gramatikova, P Christen
Jan 17, 2004·The Journal of Biological Chemistry·Stephanie PlanqueSudhir Paul

Related Concepts

Antigen-Antibody Reactions
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Enterococcus faecalis
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