The antibody to GD3 ganglioside, R24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment. Inhibitory effect of brefeldin A and monensin

The FEBS Journal
Ramiro Iglesias-BartoloméJose Luis Daniotti

Abstract

Gangliosides are sialic acid-containing glycosphingolipids present on mammalian plasma membranes, where they participate in cell-surface events such as modulation of growth factor receptors and cell-to-cell and cell-to-matrix interactions. Antibodies to gangliosides have been associated with a wide range of clinically identifiable acute and chronic neuropathy syndromes. In addition, antibodies to tumor-associated gangliosides are being used as therapeutic agents. Their binding to and release from cell membranes and intracellular destinations have not so far been extensively examined. In this study, we characterized in both GD3 ganglioside-expressing Chinese hamster ovary (CHO)-K1 and SK-Mel 28 melanoma cells the intracellular trafficking and subcellular localization of the mouse monoclonal antibody to GD3, R24. By biochemical techniques and detailed confocal microscopic analysis, we demonstrate that the GD3-R24 antibody complex is rapidly and specifically internalized by a dynamin 2-independent pathway and then accumulates in the endocytic recycling compartment. In addition, we show that the R24 antibody exits the recycling compartment en route to the plasma membrane by a dynamin 2-dependent pathway sensitive to brefeldin A and...Continue Reading

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Citations

Oct 17, 2009·The Journal of Biological Chemistry·Chen-Yu JiaoSandrine Sagan
Jul 20, 2011·The Journal of Biological Chemistry·Aldo A VilcaesJose L Daniotti
Feb 27, 2008·The Journal of Biological Chemistry·Jacqueline BoutilierPhilip A Barker
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
Jun 8, 2007·Cancer Letters·Yelena V Kovtun, Victor S Goldmacher
Mar 29, 2007·The FEBS Journal·Guillermo A Gomez, Jose L Daniotti
Jun 24, 2011·IUBMB Life·Jose Luis Daniotti, Ramiro Iglesias-Bartolomé
Nov 4, 2016·Biochimica Et Biophysica Acta. Biomembranes·Fernando M RuggieroJosé L Daniotti
Feb 14, 2007·The Journal of Biological Chemistry·Man LiangZi-Jian Xie
Jul 20, 2010·The Journal of Biological Chemistry·Pilar M CrespoJosé L Daniotti
Aug 8, 2015·The Biochemical Journal·Macarena Rodriguez-WalkerJosé L Daniotti
Sep 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nidia Alvarez-RuedaStéphane Birklé
Jan 7, 2020·Current Medicinal Chemistry·Konstantin Andreev

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