The anticalcific effect of glutaraldehyde detoxification on bioprosthetic aortic wall tissue in the sheep model

Journal of Cardiac Surgery
Peter ZillaPaul Human

Abstract

Increasing concentrations of glutaraldehyde (GA) lead to a decreased rather than increased calcification of bioprosthetic aortic wall tissue. This study determined to what extent the benefit of better cross-linking is masked by the intrinsic propensity of GA towards calcification. Porcine aortic roots were immediately fixed at the abattoir at three different concentrations of GA (0.2%, 1.0%, and 3.0% for 1 week at 4 degrees C). Subsequently, roots underwent a GA extraction process using high volumes of Urazole solution (acetic acid buffer, pH 4.5, 37 degrees C, 1 week) followed by NaBH4 reduction (2 days, 37 degrees C). Roots were implanted in the distal aortic arch of young sheep for 6 weeks and 6 months. Calcium analysis was quantitatively done by atomic absorption spectrophotometry and qualitatively assessed by light microscopy on Von Kossa stains. There was a distinct anticalcification effect of GA detoxification after 6 weeks (56.8% to 97.9%; 95% confidence interval [CI]), which stabilized on a more moderate level after 6 months of implantation (19.1% to 31.6%; 95% CI). The most pronounced effect of GA extraction was seen in 0.2% fixed tissue, where aortic wall calcification was mitigated by 97% and 32% after 6 weeks and 6...Continue Reading

References

Jan 1, 1993·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·L BengtssonA Haegerstrand
Mar 1, 1996·Biomaterials·E Jorge-HerreroJ L Castillo-Olivares
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Jan 13, 2001·The Annals of Thoracic Surgery·P ZillaU O von Oppell
Jun 5, 2001·The Annals of Thoracic Surgery·A Trantina-YatesP Zilla
Mar 30, 2002·Journal of Long-term Effects of Medical Implants·P Human, P Zilla
Oct 1, 1993·European Child & Adolescent Psychiatry·I C GillbergC Gillberg

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Citations

Jun 9, 2005·The Journal of Thoracic and Cardiovascular Surgery·Toshiharu Shin'okaHiromi Kurosawa
Dec 19, 2008·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Aiko SodaYoshihisa Yamane

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