Abstract
The anticancer effect of (1S,2S,3E,7E,11E)-3,7,11,15-cembratetraen-17,2-olide (LS-1) from Lobophytum sp. has been already reported in HT-29 human colorectal cancer cells. In this study, we examined the effect of LS-1 on the apoptosis induction of SNU-C5/5-FU, fluorouracil-resistant human colon cancer cells. Furthermore, we investigated whether the apoptosis-induction effect of LS-1 could arise from the activation of the TGF-β pathway. In SNU-C5/5-FU treated with LS-1 of 7.1 μM (IC50), we could observe the various apoptotic characteristics, such as the increase of apoptotic bodies, the increase of the sub-G1 hypodiploid cell population, the decrease of the Bcl-2 level, the increase of procaspase-9 cleavage, the increase of procaspase-3 cleavage and the increase of poly(ADP-ribose) polymerase cleavage. Interestingly, the apoptosis-induction effect of LS-1 was also accompanied by the increase of Smad-3 phosphorylation and the downregulation of c-Myc in SNU-C5/5-FU. LS-1 also increased the nuclear localization of phospho-Smad-3 and Smad-4. We examined whether LS-1 could downregulate the expression of carcinoembryonic antigen (CEA), a direct inhibitor of TGF-β signaling. LS-1 decreased the CEA level, as well as the direct interactio...Continue Reading
References
Oct 1, 1976·The Journal of Cell Biology·J FriedB D Clarkson
May 7, 1976·Analytical Biochemistry·M M Bradford
Apr 1, 1989·Experimental Cell Research·N M HooseinM G Brattain
Dec 1, 1995·The Journal of Biological Chemistry·M B DattoX F Wang
Jul 1, 1995·Journal of Natural Products·R S HouC N Lin
Dec 2, 1999·Journal of Natural Products·C Y DuhC F Dai
Mar 10, 2000·Cytokine & Growth Factor Reviews·S J KimY J Bang
Jun 28, 2000·Journal of Natural Products·C Y DuhC F Dai
May 30, 2001·Gene·G ChengJ D Lambeth
Aug 22, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·I GrivicichG Schwartsmann
Dec 12, 2001·Nature Cell Biology·Chuan-Wei JangRuey-Hwa Chen
Feb 14, 2003·International Journal of Cancer. Journal International Du Cancer·Pierpaolo CorrealeGuido Francini
Nov 20, 2004·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G MasiA Falcone
Feb 3, 2005·Oncogene·Young-Sil YoonGyesoon Yoon
Jul 13, 2006·The Journal of Cell Biology·Sylvie ThuaultAristidis Moustakas
Jan 24, 2007·Physiological Reviews·Karen Bedard, Karl-Heinz Krause
May 19, 2007·Cancer Research·Raed N SamaraJ Milburn Jessup
Jul 27, 2007·Oncogene·S-U HanS-J Kim
Sep 25, 2007·Oncogene·P Camacho-Leal, C P Stanners
Sep 26, 2007·The Journal of Biological Chemistry·Min ChenJin Wang
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Oct 29, 2008·Oncogene·K W Yip, J C Reed
Apr 28, 2010·Archives of Pharmacal Research·Huu Tung NguyenYoung Ho Kim
Oct 5, 2010·Cancer Research·Ying LiLopa Mishra
Feb 9, 2012·The Journal of Biological Chemistry·Ionica MasgrasSalvador Macip
Apr 10, 2012·Transplantation Proceedings·D Y RhyuH Ha
Jul 14, 2012·Biological & Pharmaceutical Bulletin·Ji-Young HongHee-Kyoung Kang
Oct 16, 2012·Circulation Research·Lorenzo GalluzziGuido Kroemer
Oct 24, 2012·PloS One·Soma SealSamir Bhattacharya
Feb 9, 2013·The Journal of Clinical Investigation·Neil E BholaCarlos L Arteaga
Apr 5, 2013·Marine Drugs·Min ZhaoPengcheng Yan
Jul 3, 2013·Drug Design, Development and Therapy·Hsin-chung LeeAkon Higuchi
Aug 16, 2013·Current Pharmaceutical Design·Isabel FabregatPatricia Sancho
Nov 19, 2013·Biomolecules & Therapeutics·Yhun Yhong SheenJeong-Seok Nam
Jan 5, 2014·PloS One·Daniel Nicklas, Leonor Saiz
Apr 5, 2014·Marine Drugs·Ahmed ElkhateebMohamed-Elamir F Hegazy
May 24, 2014·European Journal of Medicinal Chemistry·Sultan S Al-LihaibiFarid A Badria
Citations
Nov 1, 2016·Biomolecules & Therapeutics·Eun-Ji KimHee-Kyoung Kang
May 8, 2018·Oncotarget·Eun-Ji KimHee-Kyoung Kang
Mar 16, 2017·Natural Product Reports·John W BluntMichèle R Prinsep
Dec 22, 2016·Oncology Reports·Chen ZhangYi Wang
Nov 7, 2015·Marine Drugs·Sergey A Dyshlovoy, Friedemann Honecker
Jul 16, 2021·Journal of Ethnopharmacology·Jung Woo KimSang-Pil Yoon