Abstract
Four isoforms of actinoporins were isolated in 2002-2004 from the tropical sea anemone Heteractis crispa (=Radianthus macrodactylus). Their potent hemolytic activities and effects on Ehrlich ascites carcinoma bearing mice were also studied. In this study, the individual actinoporin (RTX-A) demonstrated potential cancer-preventive activity at extremely low and non-cytotoxic concentrations. The substance suppressed the malignant transformation of mouse JB6 P(+) Cl41 cells stimulated by epidermal growth factor (EGF) in soft agar with the inhibition of number of the colonies C(50) (INCC(50))=0.034 nM. Actinoporin RTX-A also was shown to inhibit the phenotype expression of HeLa human cancer cells with an INCC(50)=0.03 nM. The cytotoxic effect of RTX-A against JB6 P(+) Cl41 cells and HeLa, THP-1, MDA-MB-231, and SNU-C4 human tumor cell lines was high (IC(50)=0.57, 2.26, 1.11, 30.0 and 4.66 nM), but significantly less than their capacity to suppress tumor cell colony formation or phenotype expression. RTX-A also induced apoptosis and inhibited basal AP-1, NF-kappaB, and p53-dependent transcriptional activity in JB6 Cl41 cells. These results confirmed that actinoporin RTX-A from H. crispa, at least partially, might exhibit cancer-preve...Continue Reading
References
Sep 1, 1992·Cancer Metastasis Reviews·J A Hickman
Jun 1, 1992·Trends in Pharmacological Sciences·W BurschR Schulte-Hermann
May 5, 1989·Science·L R Bernstein, N H Colburn
May 15, 1989·International Journal of Cancer. Journal International Du Cancer·A D AvilaA Lage
Oct 15, 1988·International Journal of Cancer. Journal International Du Cancer·A D AvilaA Lage
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z DongN H Colburn
Nov 1, 1996·Science·A A Beg, D Baltimore
Feb 7, 1997·Cell·A J Levine
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·C HuangZ Dong
May 26, 1999·The Journal of Pathology·C Prives, P A Hall
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·M R YoungN Colburn
Dec 15, 2000·Cell·K H Vousden
Nov 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·Gregor Anderluh, Peter Macek
May 2, 2002·Science's STKE : Signal Transduction Knowledge Environment·Zigang Dong, Joan L Cmarik
Aug 8, 2002·Toxicon : Official Journal of the International Society on Toxinology·Margarita M MonastyrnayaEmma P Kozlovskaya
Jan 1, 2003·Seminars in Cancer Biology·Sharon Amit, Yinon Ben-Neriah
May 12, 2004·International Immunopharmacology·M TejucaM E Lanio
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Elena V KlyshkoEmma P Kozlovskaya
Nov 25, 2004·Progress in Biophysics and Molecular Biology·Michael W Parker, Susanne C Feil
Dec 2, 2005·Pharmaceutical Research·Sergey N FedorovZigang Dong
Oct 27, 2006·Yonsei Medical Journal·Yon Jung ParkSi Young Song
Jun 19, 2007·Cancer Research·Sergey N FedorovZigang Dong
Mar 10, 2009·Toxicon : Official Journal of the International Society on Toxinology·Carlos AlvarezMaría E Lanio
Citations
Mar 19, 2014·European Journal of Medicinal Chemistry·Dmitry N PelageevVictor Ph Anufriev
Aug 31, 2013·Cytotechnology·Mahnaz RamezanpourBarbara J S Sanderson
Nov 6, 2013·Biotechnology Letters·Mahnaz RamezanpourBarbara J S Sanderson
Jun 23, 2015·Toxins·Mahdokht JouiaeiBryan G Fry
Jun 25, 2013·Expert Opinion on Investigational Drugs·Su-Juan WangLu-Ping Qin
Jul 8, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Cui-Cui LiuYi Wang
May 31, 2011·Biochimica Et Biophysica Acta·Lucía García-OrtegaJosé G Gavilanes
Dec 24, 2011·Natural Product Reports·John W BluntMichèle R Prinsep
Jan 7, 2015·Probiotics and Antimicrobial Proteins·Nisha PonnappanArchana Chugh
Apr 30, 2016·Marine Pollution Bulletin·Luis T AnteloAntonio A Alonso
Jul 19, 2015·Toxicon : Official Journal of the International Society on Toxinology·A ValleI F Pazos
Jul 20, 2016·Marine Drugs·Sergey A DyshlovoyGunhild von Amsberg
Jan 1, 2014·Toxins·Gian Luigi Mariottini, Luigi Pane
Apr 13, 2018·Marine Drugs·Zong-Ze WuRui Chen
Oct 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei Yuen Yap, Jung Shan Hwang
Jan 24, 2019·Cell Biology International·Claudio L Q BastosRobert Tew Boyle
Nov 22, 2011·Biochemistry. Biokhimii︠a︡·E S TkachevaE P Kozlovskaya
Apr 4, 2013·Bioorganicheskaia khimiia·S A KozlovE V Grishin
Aug 2, 2012·Marine Drugs·Masashi MizunoB Paul Morgan
Jul 24, 2012·Marine Drugs·Sergey A DyshlovoyFriedemann Honecker
Apr 8, 2020·Biomolecules·Santos Ramírez-CarretoClaudia Rodríguez-Almazán
May 26, 2018·Marine Drugs·Elena LeychenkoEmma Kozlovskaya
Aug 28, 2020·Journal of Immunoassay & Immunochemistry·Sadra Samavarchi TehraniMortaza Taheri-Anganeh
Dec 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandra KvetkinaElena Leychenko
Nov 22, 2020·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Claudio Luis Quaresma Bastos JuniorRobert Tew Boyle
Aug 27, 2021·Toxins·Carlos AlvarezMaría Eliana Lanio