The anticancer effects of ferulic acid is associated with induction of cell cycle arrest and autophagy in cervical cancer cells

Cancer Cell International
Jinhua GaoYaoxian Wang

Abstract

Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a hydroxycinnamic acid derived from a rich polyphenolic compound. This study aimed to investigate the effect of ferulic acid (4-hydroxy-3-methoxycinnamic acid; FA) on cell proliferation, invasion, apoptosis, and autophagy in Hela and Caski cervical carcinoma cell lines. The cell proliferation of FA in Hela and Caski cells were detected by MTT assay. The cell invasion of FA in Hela and Caski cells were detected by Transwell assay. Subsequently, MMP-9 mRNA expression for cell invasion was detected by RT-PCR. Additionally, cell cycle and apoptosis were assayed using flow cytometry. Expression levels of 7 proteins for both cell cycle and autophagy were measured by Western blot analysis. After treated with FA (2.0 mM) for 48 h, the inhibition rates of FA in Hela and Caski cells were 88.3 and 85.4%, respectively. In addition, FA inhibited cell invasion through reducing MMP-9 mRNA expression. FA induced arrest in G0/G1 phase of the cell cycle in Hela and Caski cells with dose dependent (P < 0.05). Meanwhile, FA induced the cell cycle-related proteins expression such as p53 and p21, and reduced Cyclin D1 and Cyclin E levels. Moreover, FA decreased the autophagy-related proteins suc...Continue Reading

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Citations

Aug 2, 2019·Bioscience Reports·Madhumitha Kedhari SundaramArif Hussain
Feb 7, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Dianna LiuGansheng Zhong
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Jul 3, 2021·International Journal of Molecular Sciences·Ali MovahediQiang Zhuge
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Jan 12, 2021·Journal of Agricultural and Food Chemistry·Hao HanChing Yuan Hu

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Methods Mentioned

BETA
PCR
fluorescence-activated cell sorting

Software Mentioned

- Pro - Analyzer
ModFit
Gel

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