PMID: 12787244Jun 6, 2003Paper

The anticodon and the D-domain sequences are essential determinants for plant cytosolic tRNA(Val) import into mitochondria

The Plant Journal : for Cell and Molecular Biology
Ludovic DelageLaurence Maréchal-Drouard

Abstract

In higher plants, one-third to one-half of the mitochondrial tRNAs are encoded in the nucleus and are imported into mitochondria. This process appears to be highly specific for some tRNAs, but the factors that interact with tRNAs before and/or during import, as well as the signals present on the tRNAs, still need to be identified. The rare experiments performed so far suggest that, besides the probable implication of aminoacyl-tRNA synthetases, at least one additional import factor and/or structural features shared by imported tRNAs must be involved in plant mitochondrial tRNA import. To look for determinants that direct tRNA import into higher plant mitochondria, we have transformed BY2 tobacco cells with Arabidopsis thaliana cytosolic tRNA(Val)(AAC) carrying various mutations. The nucleotide replacements introduced in this naturally imported tRNA correspond to the anticodon and/or D-domain of the non-imported cytosolic tRNA(Met-e). Unlike the wild-type tRNA(Val)(AAC), a mutant tRNA(Val) carrying a methionine CAU anticodon that switches the aminoacylation of this tRNA from valine to methionine is not present in the mitochondrial fraction. Furthermore, mutant tRNAs(Val) carrying the D-domain of the tRNA(Met-e), although still e...Continue Reading

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Citations

Dec 17, 2008·Current Genetics·Anne-Marie DuchêneLaurence Maréchal-Drouard
Jun 2, 2005·Biochimie·Thalia SalinasAnne-Marie Duchêne
Apr 6, 2004·Molecular Biology of the Cell·Anne Crausaz EsseivaAndré Schneider
Jul 1, 2011·Nucleic Acids Research·Daria MileshinaAndré Dietrich
Jul 20, 2011·Nucleic Acids Research·Romain ValAndré Dietrich
Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Trevor Lithgow, André Schneider
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·E V SmirnovaP A Kamenski
May 23, 2007·Molekuliarnaia biologiia·P A KamenskiĭI A Tarasov
May 22, 2008·Genetics·Valérie CognatLaurence Maréchal-Drouard
Oct 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anne-Marie DuchêneIan D Small
Nov 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thalia SalinasLaurence Maréchal-Drouard
Aug 13, 2014·Nucleic Acids Research·Thalia SalinasLaurence Maréchal-Drouard
Mar 4, 2015·International Journal of Molecular Sciences·Thalia Salinas-GiegéPhilippe Giegé
Jun 3, 2008·Trends in Biochemical Sciences·Thalia SalinasLaurence Maréchal-Drouard
Sep 15, 2007·The EMBO Journal·Nabile Bouzaidi-TialiAndré Schneider
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Morgane MichaudLaurence Maréchal-Drouard
Sep 10, 2018·Biochemistry. Biokhimii︠a︡·N A VerechshaginaI O Mazunin
Sep 12, 2019·Biochemistry. Biokhimii︠a︡·T A TarasenkoYu M Konstantinov
Jun 12, 2019·IUBMB Life·Catherine Florentz, Richard Giegé

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