The anticodon loop is a major identity determinant of Saccharomyces cerevisiae tRNA(Leu)

Journal of Molecular Biology
A SomaH Himeno

Abstract

The recognition of tRNALeu, one of the class II tRNAs having a long variable arm, by leucyl-tRNA synthetase in Saccharomyces cerevisiae was studied using the T7 transcription system. Exchanging the anticodon arm of tRNALeu but not the D- or T psi C-arm to that of tRNASer seriously affected the leucine accepting activity. Two nucleotides in the anticodon loop, A35 and G37, were found to be important for leucylation. It was also found that the discriminator base, A73, is required for leucylation, and G73 of tRNASer functions as a negative identity determinant for leucyl-tRNA synthetase. Introducing a set of three base substitutions at positions 35, 37 and 73 was sufficient to convert tRNASer into an efficient leucine acceptor. These results indicate that the identity elements of tRNALeu lie at the second position of the anticodon and the 3' adjacent to the anticodon as well as the discriminator position. Such a sequence specific recognition manner is significantly different from that of Escherichia coli, in which not the anticodon but the tertiary structural elements play a key role in discriminating from other class II tRNAs. The leucine system is the first example which shows that the requirement of the anticodon sequence is va...Continue Reading

Citations

Mar 2, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Aron Szenes, Gábor Pál
Nov 4, 1998·Nucleic Acids Research·R GiegéC Florentz
Jan 16, 1999·Nucleic Acids Research·B LenhardI Weygand-Durasević
Jun 24, 2005·Nucleic Acids Research·Rui HaoEn-Duo Wang
Dec 22, 2011·Nucleic Acids Research·Kiyofumi HamashimaAkio Kanai
Mar 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ryuya FukunagaShigeyuki Yokoyama
Nov 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael J HohnDieter Söll
Apr 12, 2016·Frontiers in Microbiology·João SimõesManuel A S Santos
Apr 27, 2013·RNA Biology·Denisa D MateusManuel A S Santos
Jan 18, 2017·RNA Biology·Martin Kollmar, Stefanie Mühlhausen
Apr 8, 2014·Wiley Interdisciplinary Reviews. RNA·Yan Ling Joy PangSusan A Martinis
Feb 7, 2004·European Journal of Biochemistry·Silvija BilokapicIvana Weygand-Durasevic
Sep 15, 2004·The Journal of Biological Chemistry·Dragana KorencicDieter Söll
Apr 14, 2006·Journal of Molecular Evolution·Steven E Massey
Aug 18, 2006·Molekuliarnaia biologiia·Ana C GomesManuel A S Santos
Feb 25, 2006·Yeast·Isabel MirandaManuel A S Santos
May 23, 2019·Nucleic Acids Research·Jia-Yi FanEn-Duo Wang
Nov 10, 2011·Organic & Biomolecular Chemistry·Atsushi OgawaNobuto Matsushita
May 10, 2000·Journal of Molecular Biology·G Tocchini-ValentiniJ Abelson
Nov 5, 1999·Journal of Molecular Biology·A SomaH Himeno
Jan 1, 1996·Biochimie·M F Tuite, M A Santos
Jul 11, 2006·Journal of Molecular Biology·Ita Gruic-SovuljIvana Weygand-Durasevic
Jan 5, 2005·Biochemistry·Ekaterina I Zagryadskaya, Shana O Kelley
Nov 4, 2004·Journal of the American Chemical Society·Ning WuPeter G Schultz
Nov 10, 2021·ELife·Yekaterina Shulgina, Sean R Eddy

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