The antifungal plant defensin HsAFP1 induces autophagy, vacuolar dysfunction and cell cycle impairment in yeast.

Biochimica Et Biophysica Acta. Biomembranes
Caroline StruyfsK Thevissen

Abstract

The plant defensin HsAFP1 is characterized by broad-spectrum antifungal activity and induces apoptosis in Candida albicans. In this study, we performed a transcriptome analysis on C. albicans cultures treated with HsAFP1 to gain further insight in the antifungal mode of action of HsAFP1. Various genes coding for cell surface proteins, like glycosylphosphatidylinositol (GPI)-anchored proteins, and proteins involved in cation homeostasis, autophagy and in cell cycle were differentially expressed upon HsAFP1 treatment. The biological validation of these findings was performed in the model yeast Saccharomyces cerevisiae. To discriminate between events linked to HsAFP1's antifungal activity and those that are not, we additionally used an inactive HsAFP1 mutant. We demonstrated that (i) HsAFP1-resistent S. cerevisiae mutants that are characterized by a defect in processing GPI-anchors are unable to internalize HsAFP1, and (ii) moderate doses (FC50, fungicidal concentration resulting in 50% killing) of HsAFP1 induce autophagy in S. cerevisiae, while high HsAFP1 doses result in vacuolar dysfunction. Vacuolar function is an important determinant of replicative lifespan (RLS) under dietary restriction (DR). In line, HsAFP1 specifically r...Continue Reading

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Citations

Jan 23, 2021·Critical Reviews in Food Science and Nutrition·Laila N ShwaikiKieran M Lynch
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Jana TitsKarin Thevissen
Jan 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laila N ShwaikiElke K Arendt
Feb 22, 2021·The Journal of Biological Chemistry·Anutthaman ParthasarathyAndré O Hudson
Mar 23, 2021·Peptides·Mariana Rocha Maximiano, Octávio Luiz Franco
Apr 30, 2021·Frontiers in Cell and Developmental Biology·Caroline StruyfsKarin Thevissen
Jan 8, 2021·ACS Applied Materials & Interfaces·Jolien BreukersJeroen Lammertyn

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