PMID: 9448303Mar 14, 1998Paper

The antigen-binding characteristics of mAbs derived from in vivo priming of avian B cells

Proceedings of the National Academy of Sciences of the United States of America
N MichaelC B Thompson

Abstract

In most vertebrates, a primary antibody repertoire is created through the recombination of a diverse set of Ig variable (V), diversity (D), and joining (J) gene segments. In contrast, an avian immune repertoire is generated by gene conversion of rearranged Ig genes during B cell development within the bursa of Fabricius, a lymphoid organ unique to birds. To investigate the properties of antigen-specific Igs created through the process of gene conversion, we have developed a system for the production of avian-derived mAbs. This system was used to produce multiple antibodies after a single immunization with a conserved peptide from the human cystic fibrosis transmembrane conductance regulator gene. Each antibody isolated was found to have arisen independently through a distinct series of gene conversion events. These primary antibodies displayed evidence of diversity in all of the complementarity determining regions of both heavy and light chains, and both the heavy and the light chains contributed to antigen specificity. In the light chains, diversity could be attributed to gene conversion events. The measured affinity constants of two of the antibodies were between 10(8) and 10(9) M-1, and the antibodies were functional in quan...Continue Reading

References

Aug 1, 1991·Agricultural and Biological Chemistry·K LeeS Kaminogawa
Dec 1, 1992·Trends in Genetics : TIG·C B Thompson
May 21, 1992·Nature·E Gherardi, C Milstein
May 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M GassmannU Hübscher
Jan 24, 1991·Nature·G Winter, C Milstein
Jun 3, 1991·Journal of Immunological Methods·S NishinakaM Murata
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R ParvariI Schechter
Jun 26, 1987·Journal of Immunological Methods·J D BeattyW G Vlahos
Jan 1, 1989·International Archives of Allergy and Applied Immunology·S NishinakaM Murata
Jan 11, 1988·Nucleic Acids Research·T Simon, K Rajewsky
Nov 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·R M BauwensA P De Leenheer
Nov 20, 1987·Science·F W AltG D Yancopoulos
Jun 1, 1986·Immunological Reviews·J R Pink
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S L MorrisonV T Oi
Jan 1, 1984·American Journal of Orthodontics·D H Enlow
Oct 15, 1981·Biochemical and Biophysical Research Communications·R FertelJ R Bianchine
Jan 27, 1995·Journal of Immunological Methods·J A Woolley, J Landon

❮ Previous
Next ❯

Citations

Sep 15, 2000·Journal of Immunological Methods·J Andris-WidhopfC F Barbas
Apr 12, 2003·Drug Discovery Today·Wei-Wei Zhang
Apr 18, 2012·Protein Engineering, Design & Selection : PEDS·P J ConroyS Hearty
Aug 27, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W J J FinlayJ E Slater
Oct 1, 2004·Drug Discovery Today. Technologies·Xiangming FangWei-Wei Zhang
Feb 16, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Gregg J Silverman, Carl S Goodyear
May 11, 2004·The Journal of Veterinary Medical Science·Constantin C ConstantinoiuEiichiroh Baba
May 8, 2008·The Journal of Veterinary Medical Science·Yoshiko TateishiHaruo Matsuda
Nov 16, 2001·The Journal of Biological Chemistry·Janeen H TrembleyVincent J Kidd

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.