PMID: 6650711Sep 1, 1983

The antigenic mucin-like glycoproteins of human seminal plasma

American Journal of Reproductive Immunology : AJRI
H TortorellaA S Cerezo

Abstract

Immunodiffusion, immunoelectrophoresis, SDS-polyacrylamide gel electrophoresis, polyacrylamide gel electrophoresis, ultracentrifugation analysis, and gel permeation chromatography in chaotropic and detergent mediums showed that the human seminal plasma obtained and stored in the usual way is mainly composed by heterodispersed glycoproteins of low molecular weight. The glycoprotein components of the human seminal plasma which do not interact specifically with concanavalin A (con A) were separated by column chromatography on Con A-Sepharose according to nonspecific ion exchange and hydrophobic interactions with the protein. The carbohydrate composition of the glycoproteins and the existence of the N-acetylgalactosaminylserine (or threonine) linkage, as well as their aggregation properties show that they are mucin-type glycoproteins. They resulted "immunologically pure" although the carbohydrate chains show the structural heterodispersion usually found in mucins. The glycoproteins have a common structural pattern for the terminal nonreducing, determinant-bearing sequences of their oligosaccharide chains which, together with the low molecular weights and the known fact that mucus secretions of normal cells contain only one or two t...Continue Reading

References

Apr 1, 1977·Andrologia·G UHLENBRUCKG Steinhausen
Nov 1, 1979·Journal of Reproduction and Fertility·J M ReddyL J Zaneveld
Jan 1, 1976·Andrologia·W P Herrmann, G UHLENBRUCK
Jan 1, 1974·Annual Review of Biochemistry·S J Singer
Feb 1, 1973·Fertility and Sterility·T S Li, C G Beling
Jan 1, 1972·Archiv Für Dermatologische Forschung·W P Herrmann
Jan 29, 1973·Archiv Für Dermatologische Forschung·G UHLENBRUCKW P Herrmann
Mar 1, 1969·Journal of Reproduction and Fertility·T K Roberts, B Boettcher
Dec 1, 1971·Analytical Biochemistry·A R Johnson
Nov 1, 1968·Fertility and Sterility·L A LippielloA J Weil
May 1, 1969·Fertility and Sterility·W P Herrmann, G Hermann
Feb 1, 1966·The Journal of Endocrinology·T Mann, D A Rottenberg

Citations

Jan 1, 1987·Biomedical Chromatography : BMC·M N MazziniA S Cerezo
Aug 1, 1986·American Journal of Reproductive Immunology and Microbiology : AJRIM·M N MazziniA S Cerezo
Sep 1, 1985·Journal of Andrology·M C UsselmanD P Rossignol

Related Concepts

Neoglycoproteins
Mucins
Seminal Plasma

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