PMID: 70322Jan 1, 1977Paper

The antigenicity of soluble porcine elastins: I. Measurement of antibody by a radioimmunoassay

Connective Tissue Research
R A DaynesL Sandberg

Abstract

Immunization of rabbits with either porcine tropoelastin, lung alpha-elastin, or aortic alpha-elastin resulted in the production of antibodies against the respective antigens. The assay of antibody activity with a radioimmunoassay indicated a high degree of cross reactivity between these three soluble elastin preparations. Through the use of competitive protein binding experiments it was possible to detect antigen-specific differences between tropoelastin and the two alpha-elastins. No antigenic differences were observed between lung or aortic alpha-elastin by any of the assay procedures uded in this investigation. Absorption of the various antisera with either insolubilized tropoelastin, lung elastin or aortic elastin allowed the preparation of non-crossreactive antibodies and provided further evidence for the antigenic differences between tropoelastin and the soluble alpha-elastins. The implications of the findings, as they relate to our current understanding of the molecular and structural arrangement of elastin and the elastin precursor, are discussed.

References

Mar 1, 1975·Analytical Biochemistry·L B SandbergE G Cleary
Dec 1, 1970·Experimental Gerontology·L RobertA M Robert
Jan 1, 1973·Gerontologia·L Robert, B Robert
Aug 1, 1974·The Biochemical Journal·B C Sykes, S M Partridge
Jan 1, 1967·Methods of Biochemical Analysis·D S Jackson, E G Cleary
Jul 25, 1972·Biochemical and Biophysical Research Communications·G S David
Dec 17, 1971·Biochemical and Biophysical Research Communications·P D Weston, S Avrameas
Sep 1, 1965·The Biochemical Journal·D S JacksonE G Cleary
Apr 19, 1966·Biochemical and Biophysical Research Communications·E G ClearyD S Jackson

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Citations

Jan 1, 1981·Connective Tissue Research·J M DavidsonR G Crystal
Jun 1, 2005·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·George NicoloffPlamen Dorovski
Jan 1, 1982·Connective Tissue Research·K BarnardK G McCullagh
Mar 5, 1981·The New England Journal of Medicine·L B SandbergJ G Leslie
Mar 1, 1983·Ultrastructural Pathology·D W Urry
Jan 1, 1982·Virchows Archiv. A, Pathological Anatomy and Histopathology·J D DaviesE W Young
Jan 1, 1980·Connective Tissue Research·R P Mecham, G Lange
Aug 15, 1982·Experientia·K BarnardE W Young
Jan 1, 1981·Connective Tissue Research·U KucichG Weinbaum
Oct 1, 1982·Archives of Biochemistry and Biophysics·J M DavidsonL B Sandberg

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