PMID: 7008186Jan 1, 1980Paper

The antigens of Mycobacterium bovis, strain BCG, studied by crossed immunoelectrophoresis: a reference system

Scandinavian Journal of Immunology
O ClossM Magnusson

Abstract

A reference system for the soluble antigens of Mycobacterium bovis, strain BCG is described. The system is based on crossed immunoelectrophoresis with intermediate gel. A commercially available immunoglobulin preparation made from rabbit anti-BCG hyperimmune serum was used as reference antibodies, while a concentrated BCG culture filtrate was used as reference antigen. The pattern obtained was highly reproducible, and most of the components were stable when the fiftyfold-concentrated culture filtrate was stored at -20 degrees C. About thirty different antigenic components were selected as reference antigens and numbered. The majority of the reference antigens were present in extracts prepared from BCG by ultrasonication or bacterial press extraction. Use of the system for studies of antigenic relationship between mycobacteria, identification and quantification of antigens, and characterization of antimycobacterial antibodies are illustrated by examples. The antigens of two preparations of tuberculin purified protein derivative (PPD) were identified. The antigen designated BCG60 was found to be a najor constituent of tuberculin PPD. Evidence is presented that this antigen is cell wall associated.

References

Apr 1, 1976·Infection and Immunity·G KronvallG P Walsh
Mar 1, 1977·Bacteriological Reviews·L Barksdale, K S Kim
Jan 1, 1978·Scandinavian Journal of Immunology·M HarboeN H Axelsen
Dec 1, 1977·Infection and Immunity·M HarboeN H Axelsen
Jan 1, 1979·Scandinavian Journal of Immunology·R N MshanaM Harboe
Mar 1, 1979·Scandinavian Journal of Immunology·O ClossM Harboe
Jan 1, 1976·Scandinavian Journal of Immunology·M HarboeJ Deverill
May 1, 1973·Infection and Immunity·S D Chaparas, S R Hedrick
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
May 1, 1974·Infection and Immunity·N H AxelsenT Godal
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N H Axelsen
Jan 1, 1980·Scandinavian Journal of Immunology·E DworskyP Wetteland
Jan 1, 1964·Acta Pathologica Et Microbiologica Scandinavica·H K KIMM W BENTZON
Nov 1, 1948·The Journal of Experimental Medicine·G MIDDLEBROOK, R J DUBOS

❮ Previous
Next ❯

Citations

Jun 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L F López-CortésJ Pachón-Diaz
Jan 1, 1988·Medical Microbiology and Immunology·C Cocito, F Vanlinden
Oct 1, 1988·Journal of Biological Standardization·J NyabendaA Marchal
Jan 1, 1994·Veterinary Microbiology·V K GuptaM P Bansal
Aug 15, 1994·Veterinary Microbiology·V K GuptaM P Bansal
Aug 1, 1992·Scandinavian Journal of Immunology·H G WikerL Ljungqvist
Feb 1, 1995·Scandinavian Journal of Immunology·C Cocito, F Vanlinden
May 8, 2003·Journal of Clinical Microbiology·J M PollockP Andersen
Aug 1, 1990·Journal of Neurology, Neurosurgery, and Psychiatry·C J SindicC Cocito
Dec 18, 2007·Immunome Research·Martin J BlytheAlessandro Sette
Sep 1, 1992·European Journal of Epidemiology·M J GevaudanP De Micco
Jan 1, 1990·Chest·J P Van VoorenJ C Yernault
Feb 22, 2011·Tuberculosis·Anil K TyagiVijaya Satchidanandam
Jan 14, 2012·Scandinavian Journal of Immunology·N A LeversenT Mustafa
Dec 14, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chih-Wei LinHorng-Yunn Dou
Jun 1, 1992·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·A DrowartJ P Van Vooren
Apr 1, 1991·Clinics in Dermatology·A D Northcutt, J A Tschen
Mar 21, 1998·European Journal of Clinical Investigation·C Cocito, H Maes
Jan 1, 1994·DNA Sequence : the Journal of DNA Sequencing and Mapping·L De WitJ Content
Jan 1, 1990·Scandinavian Journal of Infectious Diseases·M C BaeldenC Cocito

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.