The antimalarial agent artesunate causes sperm DNA damage and hepatic antioxidant defense in mice

Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Supriya SinghSarbani Giri

Abstract

Artesunate is an artemisinin derivative effective against multidrug resistant malaria. We analyzed the effects of artesunate 40 mg/kg b.w. as a single dose (ART1) or 13.3mg/kg b.w. for 3 days at 24h intervals (ART2) on mice spermatozoa at morphological and molecular level, and hepatic antioxidant status following 24h and 35 days following exposures in vivo. Artesunate significantly reduced epididymal sperm count and increased the frequency of sperms with abnormal head morphology following 24h of exposure. Comet assay analysis revealed significant increase in DNA strand breaks in spermatozoa evidenced by about 3-fold increase in comet tail DNA and up to 10-fold increase in Olive tail moment following 35 days of artesunate treatment. The damage index was significantly higher in the treated groups (40.27 ± 6.62 and 37.07 ± 5.35 for ART1 and ART2 respectively) as compared to the control group (16.13 ± 3.21) indicating the genotoxic effect of artesunate. The significant reduction in GSH, SOD and increase in lipid peroxidation indicate involvement of oxidative mechanisms in artesunate induced toxicity in mice. The present study suggests that artesunate has the potential to breach the testis-blood barrier and cause toxicity to male ge...Continue Reading

References

Jun 1, 1984·Toxicology Letters· Shafiq-Ur-Rehman
Jun 1, 1995·International Journal of Radiation Biology·C R KentG G Steel
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Aug 6, 2002·Mutation Research·Sarbani GiriSurya Bali Prasad
May 17, 2003·Fertility and Sterility·Ashok AgarwalMohamed A Bedaiwy
Mar 30, 2004·Antimicrobial Agents and Chemotherapy·Sasithon PukrittayakameeNicholas J White
May 6, 2004·Basic & Clinical Pharmacology & Toxicology·Abeer F el-Nahas, Ibrahim M el-Ashmawy
Nov 5, 2004·Nature·R John AitkenSheena E M Lewis
Jan 31, 2006·Molecular and Cellular Endocrinology·Wai-Sum OP H Chow
Aug 19, 2007·Free Radical Biology & Medicine·Nicole T WattNigel M Hooper
Oct 9, 2007·Biochimica Et Biophysica Acta·Eve de Lamirande, Cristián O'Flaherty
Jul 12, 2008·Environmental and Molecular Mutagenesis·Lopamudra Das RoySarbani Giri
Jan 1, 2007·Pakistan Journal of Biological Sciences : PJBS·O A Otubanjo, A A Mosuro
Oct 15, 2009·Nigerian Journal of Physiological Sciences : Official Publication of the Physiological Society of Nigeria·A W Obianime, J S Aprioku
Sep 28, 2010·Indian Journal of Pharmacology·Bhabagrahi RathBandana Rath
Sep 1, 2003·Environmental Toxicology and Pharmacology·Suresh C JoshiTripta Sharma

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Citations

Jun 29, 2016·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Samrat DasSarbani Giri
Dec 10, 2017·Hematology·Rodrigo T Calado, Diego V Clé
May 16, 2019·Neural Regeneration Research·Bing-Wen LuLi-Ke Xie
Nov 2, 2017·Frontiers in Pharmacology·Dong-Sheng YuanQing-Ping Zeng
Jul 22, 2021·Medicinal Research Reviews·Thomas Efferth, Franz Oesch

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