The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Mariana Andrea PeraltaM Gabriela Paraje

Abstract

The antioxidant effect of 8PP, a prenylflavonoid from Dalea elegans on Candida albicans biofilms, was investigated. We previously reported that sensitive (SCa) and resistant C. albicans (RCa) biofilms were strongly inhibited by this compound, in a dose-depending manner (50 μM-100 μM), with a prooxidant effect leading to accumulation of endogenous oxidative metabolites and increased antioxidant defenses. In this work, the antifungal activity of high concentrations of 8PP (200-1000 μM), the cellular stress imbalance and the architecture of biofilms were evaluated. Biofilms were studied by crystal violet and confocal scanning laser microscopy (CSLM) with COMSTAT analysis. Superoxide anion radical, the activity of the superoxide dismutase and the total antioxidant capacity were measured. Intracellular ROS were detected by a DCFH-DA and visualized by CSLM; reactive nitrogen intermediates by Griess. An antioxidant effect was detected at 1000 μM and levels of oxidant metabolites remained low, with major changes in the SCa. COMSTAT analysis showed that biofilms treated with higher concentrations exhibited different diffusion distances with altered topographic surface architectures, voids, channels and pores that could change the flow i...Continue Reading

Citations

Jun 17, 2020·Medical Mycology·Melisa A QuinterosMaría G Paraje
Jan 19, 2021·World Journal of Microbiology & Biotechnology·Natalia I Di MarcoCarlos R Pungitore
Feb 20, 2021·Frontiers in Microbiology·María A da SilvaMaría G Paraje
Jul 28, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Julio E Arce MirandaMa Gabriela Paraje

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