The antioxidant activity of chondroitin-4-sulphate, in carbon tetrachloride-induced acute hepatitis in mice, involves NF-kappaB and caspase activation.

British Journal of Pharmacology
Giuseppe M CampoAlberto Calatroni

Abstract

Reactive oxygen species (ROC) are the main causes of carbon tetrachloride (CCl4)-induced acute liver injury. Chondroitin-4-sulphate (C4S) is known to inhibit lipid peroxidation through antioxidant mechanisms. Activation of nuclear factor (NF)-kappaB and caspases may strongly intensify inflammation and cell damage, in addition to that directly exerted by ROS. We investigated whether treatment with C4S, besides exerting antioxidant activity, was able to modulate NF-kappaB and apoptosis activation in CCl4-induced liver injury in mice. Acute hepatitis was induced in mice by an i.p. injection of CCl(4). Varying doses of C4S were administered i.p. 1 h before, 6 and 12 h after CCl4 injection. 24 h after CCl4 injection, the mice were killed for biochemical and histological analysis. CCl4 injection produced: marked elevation of alanine aminotransferase and aspartate aminotransferase; hepatic membrane lipid peroxidation, assayed by 8-isoprostane levels; and depletion of reduced glutathione and superoxide dismutase. CCl4 also decreased NF-kappaB translocation and IkBalpha, and increased gene expression of mRNA and protein of metalloproteases (MMP)-2 and -9, and of pro- and cleaved forms of caspases-3 and -7. There was also increased liver...Continue Reading

References

Sep 9, 2000·Molecular Aspects of Medicine·G Poli
Jun 8, 2001·Alcoholism, Clinical and Experimental Research·A Y SunG Y Sun
Apr 24, 2003·Critical Reviews in Toxicology·Lutz W D WeberAndreas Stampfl
Oct 14, 2003·Biochemical Pharmacology·Karen HeyninckRudi Beyaert
Nov 12, 2003·The Journal of Pharmacology and Experimental Therapeutics·Sharmilee P SawantHarihara M Mehendale
Nov 13, 2003·Experimental and Molecular Pathology·William E Stehbens
Feb 18, 2004·Current Medicinal Chemistry·Jharna BhattacharyyaAsoke G Datta
Aug 12, 2004·Free Radical Biology & Medicine·Kristin K Nelson, J Andres Melendez
Oct 7, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nicholas J Cartel, Martin Post
Dec 17, 2004·Journal of Cellular and Molecular Medicine·Wen-Xing Ding, Xiao-Ming Yin
Jan 7, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Asya RollsMichal Schwartz
Feb 1, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Harmeet MalhiJohn J Lemasters
Sep 7, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Jörn M SchattenbergMarcus Schuchmann
Sep 9, 2006·Journal of Gastroenterology and Hepatology·Dongmei WuXianglin Shi
Sep 22, 2006·Antioxidants & Redox Signaling·Cristen PantanoYvonne M W Janssen-Heininger
Dec 16, 2006·Mini Reviews in Medicinal Chemistry·G M CampoA Calatroni
Jan 20, 2007·Zeitschrift für Gastroenterologie·C Weiler-NormannA W Lohse
Jun 16, 2007·Cellular Signalling·Marcelo Genestra
Jun 27, 2007·Journal of Pharmaceutical Sciences·Tomris Ozben
Jul 7, 2007·Critical Reviews in Toxicology·T WangH M Mehendale
Feb 9, 2008·The International Journal of Biochemistry & Cell Biology·Ian M ClarkDylan R Edwards

❮ Previous
Next ❯

Citations

Aug 4, 2009·Chemical Research in Toxicology·Debra L Laskin
Nov 7, 2014·Mediators of Inflammation·Sura Wanessa Santos RochaChristina Alves Peixoto
Feb 10, 2016·Bioscience, Biotechnology, and Biochemistry·Katsumi AjisakaYasuhiro Suzuki
Nov 28, 2012·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Samuele De MinicisMarco Marzioni
Nov 30, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jie-Qiong MaChan-Min Liu
Feb 9, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hesham Farouk HasanShereen Mohamed Galal
Dec 21, 2016·International Journal of Biological Macromolecules·Zhenguo YangJie Yu
Oct 15, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Shengdong WangJie Bai
Feb 23, 2018·Experimental and Therapeutic Medicine·Chengfang YangYongwen Li
Feb 13, 2020·BMC Complementary Medicine and Therapies·Adeeb ShehzadEbtesam A Al-Suhaimi
Feb 8, 2021·Drug Discovery Today·Sarita Mishra, Munia Ganguli
Feb 10, 2020·International Journal of Biological Macromolecules·Muhammad Bilal, Hafiz M N Iqbal
Oct 6, 2009·Archives of Biochemistry and Biophysics·Giuseppe M CampoAlberto Calatroni

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis