The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.
Abstract
Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design. The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans. In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively. Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05). Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05). Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml. In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents. PRACTICAL APPLICATIONS: OS plays an important...Continue Reading
References
Antioxidative activity of water soluble polysaccharide in pumpkin fruits (Cucurbita maxima Duchesne)
Enhanced ROS production leads to excessive fat accumulation through DAF-16 in Caenorhabditis elegans
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