The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.

Journal of Food Biochemistry
Zhiyu FangYongjun Zhang

Abstract

Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design. The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans. In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively. Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05). Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05). Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml. In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents. PRACTICAL APPLICATIONS: OS plays an important...Continue Reading

References

Jul 1, 1956·Journal of Gerontology·D HARMAN
Jun 9, 2009·Bioscience, Biotechnology, and Biochemistry·Kazuhiro NaraHidenori Koga
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Jul 20, 2011·Nature Chemical Biology·Bryan C Dickinson, Christopher J Chang
Apr 4, 2015·Chinese Journal of Natural Medicines·Hong-Yan ZhuJi-Liang Xu
Aug 2, 2015·International Journal of Biological Macromolecules·Yi SongQuanhong Li
Aug 4, 2015·Redox Biology·Cristina Espinosa-DiezSantiago Lamas
Feb 6, 2017·International Journal of Biological Macromolecules·Shuang WangWeimin Wang
Jul 2, 2017·Ecotoxicology and Environmental Safety·Lingling LiJun Yan
Sep 30, 2018·International Journal of Biological Macromolecules·Aoxue LuYongjun Zhang
Nov 23, 2018·International Journal of Biological Macromolecules·Chia-Fang Chiang, Lih-Shiuh Lai
Mar 7, 2019·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Yu-Jing Huang, Guang-Xian Nan
Apr 8, 2019·International Journal of Biological Macromolecules·Jixian ZhangHaile Ma
Jul 2, 2019·International Journal of Biological Macromolecules·Mengen YuYongjun Zhang
Jul 28, 2019·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Isabelle RohnTanja Schwerdtle
Aug 11, 2019·International Journal of Biological Macromolecules· ShanChenYang Liu
Aug 12, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Chatrawee DuangjanTewin Tencomnao
Jan 1, 2020·Saudi Journal of Biological Sciences·Manal AbudawoodAli Aljohi

❮ Previous
Next ❯

Citations

Oct 15, 2020·International Journal of Molecular Sciences·Chen Zeng, Shiling Feng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.