The antioxidants of legume nodule mitochondria

Molecular Plant-microbe Interactions : MPMI
I Iturbe-OrmaetxeManuel Becana

Abstract

The mitochondria of legume root nodules are critical to sustain the energy-intensive process of nitrogen fixation. They also generate reactive oxygen species at high rates and thus require the protection of antioxidant enzymes and metabolites. We show here that highly purified mitochondria from bean nodules (Phaseolus vulgaris L. cv. Contender x Rhizobium leguminosarum bv. phaseoli strain 3622) contain ascorbate peroxidase primarily in the inner membrane (with lesser amounts detected occasionally in the matrix), guaiacol peroxidases in the outer membrane and matrix, and manganese superoxide dismutase (MnSOD) and an ascorbate-regenerating system in the matrix. This regenerating system relies on homoglutathione (instead of glutathione) and pyridine nucleotides as electron donors and involves the enzymes monodehydroascorbate reductase, dehydroascorbate reductase, and homoglutathione reductase. Homoglutathione is synthesized in the cytosol and taken up by the mitochondria and bacteroids. Although bacteroids synthesize glutathione, it is not exported to the plant in significant amounts. We propose a model for the detoxification of peroxides in nodule mitochondria in which membrane-bound ascorbate peroxidase scavenges the peroxide fo...Continue Reading

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Citations

Oct 9, 2007·Protoplasma·Vesna Hadzi-Tasković SukalovićM Vuletić
May 16, 2002·Physiologia Plantarum·Iñaki Iturbe-OrmaetxeManuel Becana
Jan 5, 2011·Antioxidants & Redox Signaling·Karl-Josef Dietz
May 27, 2005·Molecular Plant-microbe Interactions : MPMI·Jinrong WanGary Stacey
Oct 14, 2003·Plant Physiology·Manuel A MatamorosManuel Becana
Jun 13, 2006·Plant Physiology·Manuel A MatamorosManuel Becana
May 13, 2011·Plant Physiology·Alejandro Tovar-MéndezManuel Becana
Feb 27, 2013·Journal of Experimental Botany·Iker AranjueloSalvador Nogués
Apr 5, 2003·Physiologia Plantarum·Rapolu MadhusudhanHitoshi Shibata
Dec 5, 2012·The New Phytologist·Manuel A MatamorosManuel Becana
Oct 30, 2010·The Biochemical Journal·Jürke KotthausBernd Clement
Jun 5, 2013·Plant Signaling & Behavior·Adnane BargazJean-Jacques Drevon
Jul 30, 2014·Frontiers in Plant Science·Fabian Afonso-GrunzGünter Kahl
Mar 30, 2004·Plant & Cell Physiology·Le Thi-Phuong HoaShigeyuki Tajima
Mar 5, 2010·Prikladnaia biokhimiia i mikrobiologiia·A K Glian'ko, G G Vasil'eva
Mar 24, 2007·Microbiology·Cecilia I MugliaO Mario Aguilar

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