PMID: 9657991Jul 11, 1998Paper

The apeE gene of Salmonella typhimurium encodes an outer membrane esterase not present in Escherichia coli

Journal of Bacteriology
M E CarinatoC G Miller

Abstract

Salmonella typhimurium apeR mutations lead to overproduction of an outer membrane-associated N-acetyl phenylalanine beta-naphthyl ester-cleaving esterase that is encoded by the apeE gene (P. Collin-Osdoby and C. G. Miller, Mol. Gen. Genet. 243:674-680, 1994). This paper reports the cloning and nucleotide sequencing of the S. typhimurium apeE gene as well as some properties of the esterase that it encodes. The predicted product of apeE is a 69.9-kDa protein which is processed to a 67-kDa species by removal of a signal peptide. The predicted amino acid sequence of ApeE indicates that it is a member of the GDSL family of serine esterases/lipases. It is most similar to a lipase excreted by the entomopathogenic bacterium Photorhabdus luminescens. The Salmonella esterase catalyzes the hydrolysis of a variety of fatty acid naphthyl esters and of C6 to C16 fatty acid p-nitrophenyl esters but will not hydrolyze peptide bonds. A rapid diagnostic test reported to be useful in distinguishing Salmonella spp. from related organisms makes use of the ability of Salmonella to hydrolyze the chromogenic ester substrate methyl umbelliferyl caprylate. We report that the apeE gene product is the enzyme in Salmonella uniquely responsible for the hydr...Continue Reading

References

Aug 4, 1978·Molecular & General Genetics : MGG·C Heiman, C G Miller
Jul 1, 1976·Journal of Bacteriology·C G MillerC Yen
Oct 1, 1976·European Journal of Biochemistry·M PacaudG Bras
Feb 1, 1992·Journal of Clinical Microbiology·J RuizJ Gomez
Mar 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S F DeallerA L James
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M DébarbouilléG Rapoport
Oct 1, 1991·Journal of Clinical Microbiology·A M Freydiere, Y Gille
Feb 25, 1990·Nucleic Acids Research·M WatanabeT Nohmi
Jan 1, 1990·Journal of Clinical Microbiology·P M AguirreA C Velasco
Dec 10, 1971·European Journal of Biochemistry·M Pacaud, J Uriel
Jan 1, 1980·Methods in Enzymology·R Verger
May 1, 1995·Trends in Biochemical Sciences·C Upton, J T Buckley
Aug 15, 1995·Biochemical and Biophysical Research Communications·R C ChangJ F Shaw
Jun 15, 1994·Molecular & General Genetics : MGG·P Collin-Osdoby, C G Miller
Mar 1, 1994·Journal of Bacteriology·H Cho, J E Cronan
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

May 3, 2000·Journal of Applied Microbiology·K M FensterJ L Steele
May 21, 2011·Chembiochem : a European Journal of Chemical Biology·Susanne WilhelmKarl-Erich Jaeger
Feb 17, 2001·Infection and Immunity·I R Henderson, J P Nataro
Feb 20, 2007·Journal of Bacteriology·Aurelio Moraleda-Muñoz, Lawrence J Shimkets
Jun 3, 2008·Applied and Environmental Microbiology·Eva SchultheissJoachim Jose
Aug 12, 2009·The Journal of Biological Chemistry·Alicja K AntonczakEric M Tippmann
Oct 12, 2010·Chembiochem : a European Journal of Chemical Biology·Ivana Leščić AšlerKarl-Erich Jaeger
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Ian R HendersonDlawer Ala'Aldeen
Jun 20, 2019·Frontiers in Microbiology·Ina MeuskensDirk Linke
Nov 13, 1999·Journal of Bacteriology·S WilhelmK E Jaeger
Nov 1, 2005·EcoSal Plus·Ian R Henderson, James P Nataro
Nov 4, 2004·Progress in Lipid Research·Casimir C AkohJei-Fu Shaw
Jan 17, 2009·Biochemical and Biophysical Research Communications·Dong Sook LeeOhkmae K Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.