The Apical Root Canal System of Teeth with Posttreatment Apical Periodontitis: Correlating Microbiologic, Tomographic, and Histopathologic Findings

Journal of Endodontics
José F SiqueiraIsabela N Rôças

Abstract

This study evaluated the microbiological conditions of the apical root canal system of teeth with posttreatment apical periodontitis and correlated them with observations from cone-beam computed tomographic (CBCT) imaging, micro-computed tomographic (micro-CT) imaging, and histopathology. Root apices were obtained from 36 root canal-treated teeth subjected to periradicular surgery. CBCT examination was available before surgery. The apical root specimens were scanned in a micro-CT device and then cryopulverized. The powder was subjected to DNA extraction for real-time polymerase chain reaction quantification of total bacteria, Streptococcus species, members of the phylum Actinobacteria, and Enterococcus faecalis. Microbiological findings were evaluated for associations with CBCT, micro-CT, and histopathologic data. An association between lesion size and the proportion of unfilled apical canal system volume was also assessed. All cryopulverized specimens were positive for total bacteria. Actinobacteria and streptococci occurred in 35 and 33 specimens, respectively, and were usually dominant in the community. Actinobacteria counts were 2.23 times higher in granulomas than in cysts. Streptococci were significantly more present in s...Continue Reading

References

Oct 1, 1990·Journal of Endodontics·U SjogrenK Wing
Apr 1, 1990·Endodontics & Dental Traumatology·L TronstadF Cervone
Apr 20, 2001·International Endodontic Journal·R J De MoorG E Martens
Mar 27, 2003·International Endodontic Journal·E T PinheiroF J Souza-Filho
Jan 13, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·José F Siqueira, Isabela N Rôças
Apr 27, 2004·Journal of Endodontics·Isabela N RôçasKátia R N Santos
Feb 27, 2007·Journal of Endodontics·Chankhrit SathornHarold H Messer
Jun 30, 2007·Oral Microbiology and Immunology·J F SiqueiraT Guimarães-Pinto
Dec 25, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·José F Siqueira
Oct 22, 2008·Journal of Endodontics·José F Siqueira, Isabela N Rôças
May 26, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·José F Siqueira, Isabela N Rôças
Jul 2, 2009·Journal of Endodontics·Kumar Subramanian, Andre K Mickel
Oct 12, 2010·Microbial Ecology·Laurent PhilippotEllen Kandeler
Nov 22, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Domenico RicucciLarz S W Spångberg
Mar 10, 2012·Journal of Clinical Microbiology·Isabela N Rôças, José F Siqueira
Oct 6, 2014·Journal of Endodontics·Domenico RicucciIsabela N Rôças
Apr 14, 2016·Journal of Endodontics·José Claudio ProvenzanoJosé F Siqueira
Aug 9, 2016·Periodontology 2000·Magda FeresMarcelo Faveri

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Citations

Sep 2, 2020·Journal of Endodontics·Amir AzarpazhoohKenneth M Hargreaves
Oct 30, 2021·International Endodontic Journal·José F Siqueira, Isabela N Rôças
Dec 28, 2021·International Endodontic Journal·José F Siqueira, Isabela N Rôças

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