The APO-1/Fas (CD95) receptor is expressed in homozygous MRL/lpr mice

European Journal of Immunology
S M MarianiP H Krammer

Abstract

APO-1/Fas (CD95) is a transmembrane receptor that transduces apoptotic signals within various cells including T and B cells. The APO-1 gene was found to be defective in lpr mice. In these mice insertion of a retrotransposon gives rise to transcription of an abnormal mRNA and only a fraction of wild-type APO-1 mRNA. It is not clear if lpr mice still express wild-type APO-1 protein. To address this question, we prepared rabbit anti-APO-1 antibodies (Ab) with a peptide representing the extracellular sequence corresponding to residues 5-23 of APO-1. The rabbit Ab reacted with thymocytes from different mouse strains and the extent of binding was correlated with the two known APO-1 alleles. In addition, the Ab reacted with mouse cell lines expressing mouse APO-1 mRNA but not with human cell lines. Binding of the Ab to MRL and BALB/c thymocytes was completely blocked by the immunizing peptide. Immunofluorescence analysis of MRL/lpr thymocytes showed that they still express APO-1 protein at approximately one tenth of the wild-type expression level on their surface. In addition, in lpr as in wild-type mice we found a decrease of APO-1 expression in the more mature thymic compartment. Western blot analysis of whole cell lysates from lpr ...Continue Reading

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Citations

May 29, 1998·European Journal of Immunology·S M Mariani, P H Krammer
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