PMID: 2504730Sep 1, 1989Paper

The apolipoprotein A-I gene is actively expressed in the rapidly myelinating avian peripheral nerve

The Journal of Cell Biology
A C LeBlancC Mezei

Abstract

The expression of the apolipoprotein A-I (apo A-I) gene was investigated in the myelinating sciatic nerve. Hybridization analysis with an apo A-I cDNA probe obtained from a cDNA library of mRNA isolated from rapidly myelinating chick sciatic nerve indicated that apo A-I coding transcripts increase during development in the chick sciatic nerve in parallel with the increase of myelin lamellae. Substantial apo A-I-like immunoreactivity in chick sciatic nerve homogenates was detected by Western blotting. The amount of antigen increased from the 15-d embryonic stage to 1 d posthatch and then decreased. Two subcellular fractions corresponding to the cytoplasmic compartments were particularly enriched in apo A-I. apo A-I immunoreactivity was also found in highly purified myelin preparations. Immunohistochemical staining provided further evidence for the presence of apo A-I in the endoneurial compartment of the sciatic nerve. Electron microscopic examination of these fractions after negative staining showed the presence of spherical and disc-shaped particles resembling high density lipoproteins. The presence of apo A-I, cholesterol esters, phospholipids, and triacylglycerols in ultracentrifugal fractions corresponding to serum lipoprot...Continue Reading

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Citations

Jun 15, 1996·Journal of Neuroscience Research·M J LemieuxW C Breckenridge
Feb 1, 1990·Journal of Neuroscience Research·A C LeBlanc, J F Poduslo
Mar 1, 1994·Metabolic Brain Disease·A LeBlanc
Jun 1, 2005·Biochemical and Biophysical Research Communications·Michael HunterLuba Kalaydjieva
Sep 1, 1993·DNA and Cell Biology·N BhattacharyyaD Banerjee
Oct 3, 2003·Genes & Development·Mark H G VerheijenGreg Lemke
Jun 27, 2000·The Journal of Biological Chemistry·E I Posse De ChavesJ E Vance
Sep 6, 2020·Journal of Neurochemistry·Steve PedriniRalph N Martins
May 16, 1998·Protein Expression and Purification·R S KissR O Ryan

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