The apoptotic thanatotranscriptome associated with the liver of cadavers

Forensic Science, Medicine, and Pathology
Gulnaz T JavanShivani Soni

Abstract

Gene expression investigations are well-established components of ante mortem studies with broad applications ranging from elucidating basic mechanisms responsible for normal physiological processes to discovering therapeutic targets in pathophysiological conditions. However, gene expression studies and their application in the medico-legal field are still in their infancy. Therefore, the present study focuses on RNA using PCR array in the analysis of gene expression associated with tissues taken from actual criminal cases. RNA was extracted from the liver tissues of bodies with PMIs between 6 and 48 h. The results demonstrated that mRNA was stable up to 48 h postmortem. Further, as cell death is an indispensable and necessary part of the biological life cycle, apoptotic gene expression profiles were investigated. The gene expression related to the programmed cell death found in body tissues after death is defined as the apoptotic thanatotranscriptome (thanatos-, Greek for death). On comparison of control and decaying tissues, the results show that with time, pro-apoptotic genes such as caspases are up-regulated and the expression of genes responsible for anti-apoptosis such as BCL2 and BAG3 were down-regulated. Thus, this curr...Continue Reading

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Citations

Mar 5, 2016·Frontiers in Microbiology·Gulnaz T JavanJennifer G Mulle
Feb 10, 2017·Forensic Science International : Synergy·Amy E MaileDavid O Carter
Apr 22, 2020·Scientific Reports·Gulnaz T JavanJack Ballantyne
Mar 4, 2021·Forensic Science International. Genetics·Cordula HaasJack Ballantyne
Jul 2, 2019·Science & Justice : Journal of the Forensic Science Society·Paula Núñez MartínezSara C Zapico
Jun 23, 2021·Scientific Reports·Raphael Severino BonadioSilviene Fabiana de Oliveira

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