PMID: 7833668Jan 1, 1994Paper

The appearance, distribution, and longevity of receptor-[125I]T3 complexes within the nuclei of isolated rat hepatocytes

Thyroid : Official Journal of the American Thyroid Association
G L PullenK R Singh

Abstract

The nuclei of isolated rat hepatocytes were separable into three receptor compartments based upon their differential salt extractabilities: nucleoplasmic receptors (NP) extractable with 0.15 M KCl, high-salt extractable receptors (HSE) extractable with 0.4 M KCl, and salt-resistant receptors (SR) extractable with 0.4 M KCl/5 mM dithiothreitol. The receptor distribution among the three compartments was approximately NP, 45%; HSE, 30%; SR, 25%. The mean percent occupancy with endogenous T3 of the SR receptors (86%) was higher than the occupancies of the NP receptors (68%) and the HSE receptors (63%). When hepatocytes were pulsed with 3 nM [125I]T3 at 37 degrees C for brief intervals, receptor-[125I]T3 complexes were detectable in all three nuclear compartments within 15 sec. With increasing pulse intervals up to 120 sec, the receptor content of each nuclear compartment increased progressively and without evidence of preferential accumulation in any of the three compartments. To determine the life span and intercompartmental "migration" pattern of nuclear receptors, hepatocytes were pulsed with 3 nM [125I]T3 at 37 degrees C for 2.5 min or 5 min, followed by a chase with a 500-fold excess of nonlabeled T3. The population of recepto...Continue Reading

References

Jan 1, 1976·Journal of Immunological Methods·A W Wheeler, P M Hatcher
Jan 1, 1976·Methods in Cell Biology·P O Seglen
Nov 1, 1974·Experimental Cell Research·G M Williams, J M Gunn
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L J DeGrootC P Barsano

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