PMID: 9445033Jan 28, 1998Paper

The application of a homologous recombination assay revealed amino acid residues in an LTR-retrotransposon that were critical for integration

Journal of Virology
A AtwoodH L Levin

Abstract

Retroviruses and their relatives, the LTR-retrotransposons, possess an integrase protein (IN) that is required for the insertion of reverse transcripts into the genome of host cells. Schizosaccharomyces pombe is the host of Tf1, an LTR-retrotransposon with integration activity that can be studied by using techniques of yeast genetics. In this study, we sought to identify amino acid substitutions in Tf1 that specifically affected the integration step of transposition. In addition to seeking amino acid substitutions in IN, we also explored the possibility that other Tf1 proteins contributed to integration. By comparing the results of genetic assays that monitored both transposition and reverse transcription, we were able to seek point mutations throughout Tf1 that blocked transposition but not the synthesis of reverse transcripts. These mutant versions of Tf1 were candidates of elements that possessed defects in the integration step of transposition. Five mutations in Tf1 that resulted in low levels of integration were found to be located in the IN protein: two substitutions in the N-terminal Zn domain, two in the catalytic core, and one in the C-terminal domain. These results suggested that each of the three IN domains was requi...Continue Reading

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Citations

Nov 12, 2005·Journal of Virology·Angela Atwood-MooreHenry L Levin
Aug 19, 2007·PLoS Genetics·Alfica SehgalPeter J Espenshade
Sep 26, 2015·Science·Jake Z JacobsMikel Zaratiegui
Mar 4, 2008·Cell Research·Kathryn A O'Donnell, Jef D Boeke
Feb 23, 2020·PLoS Genetics·Wioletta CzajaCasey M Bergman
Nov 30, 2012·Nucleic Acids Research·Gang FengHenry L Levin
Dec 26, 2008·Journal of Virology·Atreyi Ghatak ChatterjeeHenry L Levin
Oct 10, 1998·Journal of Virology·S J Steele, H L Levin
Dec 14, 2018·Genome Research·Caroline EsnaultHenry L Levin

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