PMID: 9536433Apr 16, 1998Paper

The application of human monoclonal antibodies for monitoring donor derived soluble HLA class I molecules in the serum of heart transplant recipients

Human Immunology
C A KoelmanF H Claas

Abstract

Increased levels of both donor and recipient derived HLA molecules can be found in serum and plasma of transplanted patients during rejection. Recent data suggest that levels of donor specific soluble HLA Class I (sHLA-1) correlate better with graft rejection than total sHLA Class I [1, 2]. Therefore, quantification of donor specific soluble counterparts of HLA Class I in the serum of the recipient may be a new way for non-invasive monitoring of rejection after organ transplantation. Up to now, only a limited number of mouse monoclonal antibodies (alpha HLA-A2, and alpha HLA-B7) has been used in enzyme linked immunosorbent assays (ELISAs) to detect donor specific HLA molecules in the plasma of transplant recipients. To monitor other donor-recipient combinations, we tested some of our HLA Class I specific human monoclonal antibodies, routinely used in complement dependent cytotoxicity, for their suitability in ELISA based assays. In the present model system, we used alpha HLA-A9 (BvK5C4) or alpha HLA-A3 (OK2F3) hybridoma-supernatant to set up a sHLA-A9 and sHLA-A3 specific ELISA. In a pilot study we show that these assays were sensitive enough to detect an increase of donor specific sHLA-I during rejection in the plasma of two h...Continue Reading

References

Jan 1, 1992·Journal of Neuroimmunology·J C Alvarez-CermeñoP González-Porqué
Dec 1, 1990·Journal of the Neurological Sciences·J C Alvarez-CermeñoP Gonzalez-Porque
Mar 1, 1989·Transplantation·H S DaviesR Y Calne
May 31, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·A FerreiraP González-Porqué
Aug 1, 1982·Human Immunology·S A EllisA McMichael
Sep 1, 1994·Transplant Immunology·C J WuC Clayberger
Jan 1, 1996·Immunological Investigations·D M ChangJ Wei

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Citations

Mar 26, 2004·Human Immunology·Melanie L MolitorWilliam J Burlingham
Jul 17, 1999·Human Immunology·A MinguelaM R Alvarez-López
Jun 3, 1999·The New England Journal of Medicine·E C GuinanJ G Gribben

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