PMID: 107930Feb 1, 1979

The application of immobilized enzymes in an artificial liver support system

Artificial Organs
G BrunnerH Lösgen

Abstract

A novel method of extracorporeal support for fulminant liver failure is reported whereby the most important detoxification processes of the liver are reproduced in an enzyme reactor. Most of the endogenous toxins involved in hepatic coma can be deactivated directly by conjugation with a hydrophilic residue such as glucuronic acid or glutathione, or by the neutralization of active groups through structural modification by methyl transfer. The enzymes responsible for these processes have been isolated and purified from rabbit liver, and covalently bound onto a hemocompatible form of agarose matrix. This system has been shown to be capable of catalyzing the desired reactions with endogenous toxins such as phenols and mercaptans in vitro, and phenols in rabbits in vivo.

References

Jan 1, 1975·Annual Review of Medicine·L Zieve, D M Nicoloff
Jan 1, 1978·Biomaterials, Medical Devices, and Artificial Organs·H LösgenA Siehoff
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·P CuatrecasasC B Anfinsen

Citations

May 20, 1996·Biotechnology and Bioengineering·A KamlotA A Demetriou
Sep 25, 2002·Transplantation Proceedings·A G R Sheil
Jun 28, 2005·American Journal of Nephrology·Vincent Launay-VacherGilbert Deray
Sep 1, 1991·Digestive Diseases and Sciences·T TakahashiY Nosé
Jan 1, 1995·Scandinavian Journal of Gastroenterology. Supplement·A A DemetriouH Rosen
Jul 31, 1999·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·V SegalN Lotan
Aug 24, 2006·Xenotransplantation·J Rozga
Jun 2, 2001·Clinics in Liver Disease·A SechserR W Osorio
Jan 1, 1981·Archives of Dermatological Research·Z HanickiJ Czabanowska
May 1, 1979·Artificial Organs·K D Kulbe
Mar 25, 1994·Biotechnology and Bioengineering·J RozgaT Giorgio
Jun 1, 1992·Artificial Organs·P KonstantinG Brunner

Related Concepts

Acamol
Artificial Organs
Glucuronic Acids
Hemoperfusion
Liver
Metabolic Detoxication, Drug
Sulfhydryl Compounds
UDP-Glucuronic Acid 3-O-beta-D-Galactosyl-D-Galactose Glucuronosyltransferase

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