The application of immunoassay techniques, including enzyme-linked immunosorbent assay (ELISA), to snake venom research

Toxicon : Official Journal of the International Society on Toxinology
R D G Theakston

Abstract

The development and application of immunoassay techniques in relation to snake venom research is reviewed. Enzyme linked immunosorbent assay (ELISA) is compared with radioimmunoassay, immunodiffusion, immunofluorescence, haemagglutination and immunoelectrophoresis. It is concluded that ELISA is the most versatile immunoassay technique so far applied to the field of venom research, its main advantages over other methods including relatively high levels of sensitivity and specificity, reproducibility, simplicity and ease of sample collection. It can also be readily modified into kit form and is easily adapted for use in large scale epidemiological studies and for accurate retrospective diagnosis of snake bite. None of the other assay systems considered fulfil these criteria to the same extent. ELISA is helping to advance epidemiological knowledge of snake bite, in exploring the role of active immunisation and in the compilation of accurate clinical patterns of envenoming. Other applications of the test include its use for potency screening of both new and developed commercially available antivenoms and for the detection of monoclonal antibodies which should eventually result in increased specificity of the assay system by elimina...Continue Reading

References

Jan 27, 1979·Lancet·S K SutherlandR D Harris
Sep 22, 1979·Lancet·R N PughH A Reid
Jan 1, 1979·Toxicon : Official Journal of the International Society on Toxinology·R D Theakston, H A Reid
Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·M L McLarenJ E Lillywhite
Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·J W LarrickJ Kaplan
Jan 1, 1976·Journal of Immunological Methods·J W Goding
Jan 1, 1976·Transactions of the Royal Society of Tropical Medicine and Hygiene·A Bartlett, D E Bidwell
Nov 3, 1975·Biochemical and Biophysical Research Communications·J Gow, A C Wardlaw
Dec 28, 1974·British Medical Journal·B M GreenwoodH A Reid
Mar 1, 1974·Journal of Immunological Methods·A R CoulterA J Broad
Nov 1, 1969·Toxicon : Official Journal of the International Society on Toxinology·E R Trethewie, W L Thomas
Jun 1, 1972·Toxicon : Official Journal of the International Society on Toxinology·E KarlssonL Rydén
Oct 1, 1968·Toxicon : Official Journal of the International Society on Toxinology·R D Boche, F E Russell
May 20, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·H M Chandler, J G Hurrell
Jan 1, 1958·International Archives of Allergy and Applied Immunology·A B STAVITSKY, E R ARQUILLA

❮ Previous
Next ❯

Citations

Nov 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·R D Theakston
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·R D TheakstonR R New
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·M HoA Voller
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·D IddonR D Theakston
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·H LabrousseS Avrameas
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·L ChinonavanigK Ratanabanangkoon
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·K K TanW A Ariffin
Dec 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·N H TanM I Jaafar
May 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·F AudebertC Bon
Apr 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·C Chavez-OlorteguiC R Diniz
Mar 1, 1995·Toxicon : Official Journal of the International Society on Toxinology· Tun-Pe May-Mya-Win
Oct 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·M P ReveloC Chávez-Olórtegui
Jan 1, 1985·Pharmacology & Therapeutics·A Ménez
Mar 30, 2004·Biosensors & Bioelectronics·Le Van DongP Gopalakrishnakone
Jun 22, 2002·Toxicon : Official Journal of the International Society on Toxinology·C Maya DeviL K Krishnan
Mar 27, 2003·Toxicon : Official Journal of the International Society on Toxinology·Dong-Zong HungShoei-Yn Lin-Shiau
Mar 27, 2003·Toxicon : Official Journal of the International Society on Toxinology·R A HarrisonR D G Theakston
May 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·C F AmaralN A Rezende
Jul 15, 1998·Toxicon : Official Journal of the International Society on Toxinology·J P Chippaux, M Goyffon
Mar 19, 1999·Toxicon : Official Journal of the International Society on Toxinology·Z E Selvanayagam, P Gopalakrishnakone
Apr 29, 1999·Toxicon : Official Journal of the International Society on Toxinology·Z E SelvanayagamP V Rao
Aug 23, 2002·Biosensors & Bioelectronics·Z Emmanuel SelvanayagamL C Ho
Sep 1, 1993·Journal of Immunoassay·L Kittigul, K Ratanabanangkoon
May 27, 2014·Toxins·R David G Theakston, Gavin D Laing
Jun 21, 2014·Toxicon : Official Journal of the International Society on Toxinology·Po-Shun Chuang, Jen-Chieh Shiao
Dec 19, 2015·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Mark MillerGeoffrey K Isbister
Jun 28, 2008·Toxicon : Official Journal of the International Society on Toxinology·A F Wahby, N M Ibrahim
Jan 22, 2008·Toxicon : Official Journal of the International Society on Toxinology·Carlos R de MedeirosFábio F M Castro
Jan 4, 2008·British Journal of Clinical Pharmacology·Geoffrey K IsbisterJennifer S Schneider
Jun 13, 2006·Toxicon : Official Journal of the International Society on Toxinology·Margaret A O'LearyBart J Currie
Sep 1, 1987·Annals of Emergency Medicine·S A Minton
Jan 1, 1991·Journal of Toxicology. Clinical Toxicology·L ChinonavanigK Ratanabanangkoon
Nov 11, 2003·The Journal of Pharmacy and Pharmacology·Liu XingShao Lan
Nov 21, 2013·Toxicon : Official Journal of the International Society on Toxinology·M A O'Leary, G K Isbister
Dec 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·Margaret A O'LearyGeoffrey K Isbister
Jun 28, 2016·Talanta·Holger HoffmannRudolf J Schneider
Jul 6, 2016·Toxicon : Official Journal of the International Society on Toxinology·Balasaheb S PawadeMugdha Potnis-Lele

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicon : Official Journal of the International Society on Toxinology
M HoA Voller
Toxicon : Official Journal of the International Society on Toxinology
Z E Selvanayagam, P Gopalakrishnakone
Toxicon : Official Journal of the International Society on Toxinology
S PukrittayakameeD Bunnag
© 2021 Meta ULC. All rights reserved