The application of laser scanning confocal microscopy to the examination of hairs and textile fibers: an initial investigation

Forensic Science International : Synergy
K Paul Kirkbride, Silvana R Tridico

Abstract

An initial investigation of the application of laser scanning confocal microscopy to the examination of hairs and fibers has been conducted. This technique allows the production of virtual transverse and longitudinal cross-sectional images of a wide range of hairs and fibers. Special mounting techniques are not required; specimens that have been mounted for conventional microscopy require no further treatment. Unlike physical cross-sectioning, in which it is difficult to produce multiple cross-sections from a single hair or fiber and the process is destructive, confocal microscopy allows the examiner to image the cross-section at any point in the field of view along the hair or fiber and it is non-destructive. Confocal microscopy is a fluorescence-based technique. The images described in this article were collected using only the autofluorescence exhibited by the specimen (i.e. fluorescence staining was not necessary). Colorless fibers generally and hairs required excitation at 405 nm in order to stimulate useful autofluorescence; longer wavelength excitation was suitable for dyed fibers. Although confocal microscopy was found to be generally applicable to the generation virtual transverse cross-sections from a wide range of ha...Continue Reading

References

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Citations

Aug 27, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Hilda M Hernández-HernándezReynold R Farrera-Rebollo
Jun 20, 2018·Veterinary Dermatology·Paweł TumiłowiczMarcin Szczepanik
May 16, 2020·Forensic Science, Medicine, and Pathology·Janet Davey, Gale Spring
May 3, 2011·Analytical Chemistry·T A BrettellJ R Almirall
Mar 23, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Wataru TadanoShinjiro Hayakawa

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