The application of novel 1.7 microm ethylene bridged hybrid particles for hydrophilic interaction chromatography

Journal of Separation Science
Eric S GrumbachUwe D Neue

Abstract

An un-derivatized 1.7 microm ethylene bridged hybrid (BEH) particle was evaluated for its utility in retaining polar species in hydrophilic interaction chromatography (HILIC), and was compared to a 3 microm un-derivatized silica material. Retentivity as a function of mobile phase pH, polar modifier and ACN content was examined. Also, the efficiency of the two particle substrates was compared by plotting HETP vs. linear velocity. Improved chemical resistance of the un-derivatized BEH particle was compared to un-derivatized silica at pH 5, demonstrating no performance deterioration over the course of 2000 injections for the BEH particle, while the silica particle deteriorated rapidly after 800 injections. Lastly, ESI-MS sensitivity as a function of particle size and separation mode was demonstrated. A 2.2 to 4.7-times higher ESI-MS response was observed on the 1.7 microm particle compared to the 3 microm particle, whereas a 5.6 to 8.8-times higher ESI-MS response was observed using HILIC as when compared to traditional RP chromatography.

References

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Apr 2, 2003·Journal of Pharmaceutical and Biomedical Analysis·Yong Guo, Aihua Huang
Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Weng Naidong
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Sep 15, 2006·Journal of Separation Science·Petrus Hemström, Knut Irgum
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Citations

Oct 27, 2012·Nature Protocols·Serena Di PalmaAlbert J R Heck
Dec 15, 2010·Biomedical Chromatography : BMC·Lewis Couchman, Phillip E Morgan
Apr 4, 2017·Electrophoresis·Lucas VeillonYehia Mechref
Jun 29, 2011·Journal of Separation Science·Nilusha L T Padivitage, Daniel W Armstrong
Nov 12, 2009·Chemical Communications : Chem Comm·Hongxue HuangXinmiao Liang
Dec 31, 2009·Journal of Separation Science·Uwe D NeuePamela C Iraneta
Feb 26, 2010·Journal of Separation Science·Bénédicte ChauveJean-Luc Veuthey
Feb 16, 2010·Journal of Separation Science·Wenying JianNaidong Weng
Mar 12, 2010·Journal of Separation Science·Giorgio MarrubiniGabriella Massolini
Apr 16, 2010·Analytical and Bioanalytical Chemistry·Spyros DrivelosNikolaos S Thomaidis
Sep 6, 2019·Medicines·Patricia ReganMargaret Doherty
Jun 9, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lana Abou ZeidCarole Bresson
Jul 5, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nikunj TannaRobert S Plumb

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