The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock

Veterinary Parasitology
R A MasakeP A O Majiwa

Abstract

Teneral tsetse flies infected with either Trypanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infected tsetse developed a fluctuating parasitaemia. DNA was extracted from the blood of these cattle and subjected to polymerase chain reaction (PCR) using oligonucleotide primers specific for T. brucei or T. vivax. The PCR products unique to either T. brucei or T. vivax were identified following amplification of DNA from the blood samples of infected cattle, whereas none was detectable in the DNA from the blood of the cattle exposed to non-infected teneral tsetse. In a concurrent set of experiments, one of the oligonucleotide primers in each pair was biotinylated for use in PCR-ELISA to examine all the blood samples with this assay. Both the PCR and the PCR-ELISA revealed trypanosome DNA in 85% of blood samples serially collected from the cattle experimentally infected with T. brucei. In contrast, the parasitological assays showed trypanosomes in only 21% of the samples. In the blood samples from cattle experimentally infected with T. viva...Continue Reading

References

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Citations

Oct 7, 2003·International Journal for Parasitology·J E HodgkinsonJ B Matthews
Jul 24, 2003·Trends in Microbiology·Eddy C AgboMarinus F W te Pas
Jul 24, 2007·Experimental Parasitology·Kim PicozziSusan C Welburn
Aug 11, 2004·Acta Tropica·Yvonne GallPeter-Henning Clausen
Jun 3, 2015·Parasite : Journal De La Société Française De Parasitologie·Vincent DjohanPhilippe Solano
Jun 1, 2006·Advances in Parasitology·E M FèvreI Maudlin

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