PMID: 1206223Dec 1, 1975Paper

The application of radiolabelled HL-A antibodies in the detection of (soluble) transplantation antigens: an alternative for the microcytotoxicity test

Journal of Immunological Methods
M J GiphartJ J Rood

Abstract

The use of radiolabelled anti HL-A antibody preparations is described. Absorption by platelets, followed by acid elution, fractionation of the eluate by column chromatography, radiolabelling and a second absorption--elution step with platelets yielded specific preparations, irrespective of original cytotoxic titres. The binding of these radiolabelled antibodies to lymphocytes could be inhibited by soluble antigens present in normal plasma or obtained from spleen cells by papain treatment. As a method for the detection of soluble transplantation antigens the use of radiolabelled antibodies appeared as sensitive as the microcytotoxicity test.

Citations

Jun 30, 2005·Rheumatology International·Irena AdamashviliJohn C McDonald
Jul 31, 1998·Human Immunology·J C McDonald, I Adamashvili
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D B JarrettJ S Flier
Aug 27, 1997·Transplantation·J C McDonald
Oct 22, 2008·Fertility and Sterility·Mohamed A BedaiwyTommaso Falcone
Jan 1, 1976·Transplantation Reviews·J J van RoodJ J Keuning
Dec 1, 1979·Clinical Immunology and Immunopathology·R K SchuurmanH K Visser

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