The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens

BMC Microbiology
Beata DolkaPiotr Szeleszczuk

Abstract

Enterococcus hirae is considered a part of the normal intestinal biota of several domestic animals, including poultry. However, this species is also associated with infective endocarditis in chickens, a disease that leads to unexpected deaths and serious economical losses. Enterococcus hirae is identified predominantly with the use of conventional bacteriological methods, biochemical tests and PCR. Rapid, sensitive and specific methods for detecting E. hirae in clinical samples are required in poultry production. The aim of this study was to use the Loop-Mediated Isothermal Amplification (LAMP) for the identification and quantification of E. hirae in heart samples from broiler chickens. The specificity of the LAMP method was confirmed for 7 enterococcal strains and 3 non-enterococcal strains. E. hirae was detected in all of the 22 analyzed clinical bacterial isolates and in all of the 9 heart samples. Three sets of primers supported the detection of E. hirae with high sensitivity and specificity within one hour. The highest detection rate of a LAMP product was approximately 7 min for an E. hirae strain and 12 min for a positive heart sample. The detection limit for the E. hirae ATCC 10541 standard was 1.3 × 102 CFU (43.4 fg) or...Continue Reading

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Citations

Aug 23, 2020·Animals : an Open Access Journal From MDPI·Gustaw M SzafraniecBeata Dolka
Jul 27, 2021·Radiology Case Reports·Rahul BollamTung Phan

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Datasets Mentioned

BETA
EU02133
MG581168
MG581169

Methods Mentioned

BETA
PCR
electrophoresis
nucleic acid amplification
Fluorescence
PCRs

Software Mentioned

BLAST Sequence Analysis Tool
LAMP Designer
Excel
Genomed

Related Concepts