The application of transcription activator-like effector nucleases for genome editing in C. elegans

Methods : a Companion to Methods in Enzymology
Peishan YiGuangshuo Ou

Abstract

The nematode Caenorhabditis elegans has been a powerful model system for biomedical research in the past decades, however, the efficient genetic tools are still demanding for gene knockout, knock-in or conditional gene mutations. Transcription activator-like effector nucleases (TALENs) that comprise a sequence-specific DNA-binding domain fused to a FokI nuclease domain facilitate the targeted genome editing in various cell types or organisms. Here we summarize the recent progresses and protocols using TALENs in C. elegans that generate gene mutations and knock-ins in the germ line and the conditional gene knockout in somatic tissues.

References

May 31, 2014·Methods : a Companion to Methods in Enzymology·E Jane Albert Hubbard

Citations

Nov 26, 2009·Science·Matthew J Moscou, Adam J Bogdanove
Mar 23, 2011·Nucleic Acids Research·Robert MorbitzerThomas Lahaye
Jun 14, 2012·Nucleic Acids Research·Erin L DoyleAdam J Bogdanove
Nov 1, 2012·Science·Jennifer L GarrisonCornelia I Bargmann
Feb 5, 2013·Nature Methods·Pablo Perez-PineraCharles A Gersbach
Jul 24, 2013·Nucleic Acids Research·Tomonori KatsuyamaRenato Paro
Aug 28, 2013·Genetics·Hui ChiuPaul W Sternberg
Aug 28, 2013·Genetics·Selma WaaijersMike Boxem
Sep 5, 2013·Nucleic Acids Research·Florian HeigwerMichael Boutros

Related Concepts

Calcinus elegans
Guanine
Embryo
Cyartonema elegans
Coleonyx elegans
Non-Homologous DNA End-Joining
Cestrum elegans
Clarkia unguiculata
Clathrulina elegans
Cardioglossa elegans

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