The Applications of Promoter-gene-Engineered Biosensors

Sensors
Yingzhu FengJianzhong Liu

Abstract

A promoter is a small region of a DNA sequence that responds to various transcription factors, which initiates a particular gene expression. The promoter-engineered biosensor can activate or repress gene expression through a transcription factor recognizing specific molecules, such as polyamine, sugars, lactams, amino acids, organic acids, or a redox molecule; however, there are few reported applications of promoter-enhanced biosensors. This review paper highlights the strategies of construction of promoter gene-engineered biosensors with human and bacteria genetic promoter arrays with regard to high-throughput screening (HTS) molecular drugs, the study of the membrane protein's localization and nucleocytoplasmic shuttling mechanism of regulating factors, enzyme activity, detection of the toxicity of intermediate chemicals, and probing bacteria density to improve value-added product titer. These biosensors' sensitivity and specificity can be further improved by the proposed approaches of Mn2+ and Mg2+ added random e error-prone PCR that is a technique used to generate randomized genomic libraries and site-directed mutagenesis approach, which is applied for the construction of bacteria's "mutant library". This is expected to est...Continue Reading

References

Feb 26, 2008·Journal of Bioscience and Bioengineering·Takuo HasegawaTsuyoshi Nakamatsu
Jul 9, 2008·Metabolic Engineering·Nikolaos AnesiadisRadhakrishnan Mahadevan
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kechun ZhangJames C Liao
Apr 7, 2010·Annual Review of Biochemistry·Jeffrey A DietrichJay D Keasling
Jan 27, 2011·Angewandte Chemie·Shuang-Yan Tang, Patrick C Cirino
Jun 8, 2012·The New England Journal of Medicine·Yanlin ZhaoYu Wang
Feb 19, 2013·Methods in Molecular Biology·Shuobo ShiXueming Zhao
May 10, 2013·ACS Synthetic Biology·Nikolaos AnesiadisRadhakrishnan Mahadevan
Aug 15, 2013·Analytical Chemistry·Shaohui HuHeng Zhu
Oct 22, 2013·Nature Biotechnology·Robert H DahlJay D Keasling
Jan 17, 2015·Nature Reviews. Cancer·Toshiro MoroishiKun-Liang Guan
Nov 2, 2015·Applied Microbiology and Biotechnology·Regina Mahr, Julia Frunzke
Jan 24, 2017·Metabolic Engineering·Dandan XiongShuang-Yan Tang
Jan 25, 2017·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Md Mahmudul IslamTianyu Zhang
Jan 19, 2018·Journal of Industrial Microbiology & Biotechnology·Zhen LiJian-Zhong Liu
Mar 20, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Cláudia S NóbregaSofia R Pauleta
Jul 7, 2018·Current Opinion in Organ Transplantation·Christine Koval

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensors
PCR
fluorescence-activated cell sorting
chip
Biosensor
chips

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.