The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction

The Plant Cell
J LeungJ Giraudat

Abstract

Abscisic acid (ABA) mediates seed maturation and adaptive responses to environmental stress. In Arabidopsis, the ABA-INSENSITIVE1 (ABI1) protein phosphatase 2C is required for proper ABA responsiveness both in seeds and in vegetative tissues. To determine whether the lack of recessive alleles at the corresponding locus could be explained by the existence of redundant genes, we initiated a search for ABI1 homologs. One such homolog turned out to be the ABI2 locus, whose abi2-1 mutation was previously known to decrease ABA sensitivity. Whereas abi1-1 is (semi)dominant, abi2-1 has been described as recessive and maternally controlled at the germination stage. Unexpectedly, the sequence of the abi2-1 mutation showed that it converts Gly-168 to Asp, which is precisely the same amino acid substitution found in abi1-1 and at the coincidental position within the ABI1 phosphatase domain (Gly-180 to Asp). In vitro assays and functional complementation studies in yeast confirmed that the ABI2 protein is an active protein phosphatase 2C and that the abi2-1 mutation reduced phosphatase activity as well as affinity to Mg2+. Although a number of differences between the two mutants in adaptive responses to stress have been reported, quantitati...Continue Reading

References

Oct 1, 1992·The Plant Cell·J GiraudatH M Goodman
Dec 1, 1991·Plant Molecular Biology·S J Gilmour, M F Thomashow
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Oct 25, 1989·Nucleic Acids Research·W J Shen, B G Forde
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Aug 1, 1993·The Plant Journal : for Cell and Molecular Biology·A Konieczny, F M Ausubel
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·A SchwartzS M Assmann
May 1, 1993·Trends in Biochemical Sciences·M J Hubbard, P Cohen
Nov 1, 1995·The Plant Journal : for Cell and Molecular Biology·F CreusotA Durr
Aug 1, 1996·The Plant Journal : for Cell and Molecular Biology·E SödermanP Engström
Aug 1, 1996·Trends in Biochemical Sciences·M C Faux, J D Scott
Jun 1, 1996·Plant Physiology·G L de BruxellesR Dolferus
Oct 1, 1996·European Journal of Biochemistry·N BertaucheJ Giraudat

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Citations

Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock
Mar 29, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Molly Cadle-Davidson, Molly M Jahn
Jul 13, 2006·Plant Cell Reports·Kandakumar JayasekaranSung Han Ok
Apr 11, 2013·Plant Cell Reports·Jing Han HongJian Xu
Jul 9, 2008·Molecular Genetics and Genomics : MGG·Melissa S HillwigGustavo C Macintosh
Dec 1, 2005·Functional & Integrative Genomics·Chitra RaghavanTrevor W Stevenson
Mar 19, 2011·Journal of Plant Research·Daisuke TakezawaYoichi Sakata
Sep 11, 2007·Plant Molecular Biology·Hongmei LiChuanyou Li
Feb 19, 2008·Plant Molecular Biology·Bas J W DekkersSjef C M Smeekens
Nov 26, 2008·Plant Molecular Biology·Biswa R Acharya, Sarah M Assmann
Oct 31, 2012·Plant Molecular Biology·Shuxin ZhangPieter B F Ouwerkerk
Jun 21, 2013·Plant Molecular Biology·Xiao-Feng ZhangDa-Peng Zhang
Aug 13, 2013·Plant Molecular Biology·Ge BaiJian-Kang Zhu
Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Vijaya Shukla, Autar K Mattoo
Feb 12, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·P LessardM Thomas
Oct 12, 2001·Trends in Endocrinology and Metabolism : TEM·C Müssig, T Altmann
Mar 3, 2001·Current Opinion in Plant Biology·N Bouché, D Bouchez
Sep 16, 2003·Current Opinion in Plant Biology·Josef M Kuhn, Julian I Schroeder
Mar 6, 1999·Current Opinion in Plant Biology·E Grill, A Himmelbach
Mar 6, 1999·Current Opinion in Plant Biology·R Walden
Oct 6, 1999·Current Opinion in Plant Biology·E Nambara, P McCourt
Aug 1, 2013·Nature Communications·Kenji KomatsuYoichi Sakata
Mar 20, 2003·Cell Research·Fang LinHong Wei Xue
Feb 14, 2002·Plant, Cell & Environment·S. Luan

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