The Arabidopsis AtPP2CA Protein Phosphatase Inhibits the GORK K+ Efflux Channel and Exerts a Dominant Suppressive Effect on Phosphomimetic-activating Mutations.

The Journal of Biological Chemistry
Cécile LefoulonI Cherel

Abstract

The regulation of the GORK (Guard Cell Outward Rectifying) Shaker channel mediating a massive K(+) efflux in Arabidopsis guard cells by the phosphatase AtPP2CA was investigated. Unlike the gork mutant, the atpp2ca mutants displayed a phenotype of reduced transpiration. We found that AtPP2CA interacts physically with GORK and inhibits GORK activity in Xenopus oocytes. Several amino acid substitutions in the AtPP2CA active site, including the dominant interfering G145D mutation, disrupted the GORK-AtPP2CA interaction, meaning that the native conformation of the AtPP2CA active site is required for the GORK-AtPP2CA interaction. Furthermore, two serines in the GORK ankyrin domain that mimic phosphorylation (Ser to Glu) or dephosphorylation (Ser to Ala) were mutated. Mutations mimicking phosphorylation led to a significant increase in GORK activity, whereas mutations mimicking dephosphorylation had no effect on GORK. In Xenopus oocytes, the interaction of AtPP2CA with "phosphorylated" or "dephosphorylated" GORK systematically led to inhibition of the channel to the same baseline level. Single-channel recordings indicated that the GORK S722E mutation increases the open probability of the channel in the absence, but not in the presence...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·F ArmstrongM R Blatt
Feb 1, 1995·The Plant Journal : for Cell and Molecular Biology·A A VéryJ B Thibaud
Jan 1, 1995·Methods in Enzymology·P L Bartel, S Fields
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·I Marten, T Hoshi
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Sheen
Feb 22, 2000·FEBS Letters·B LacombeJ B Thibaud
Apr 5, 2000·Biochimica Et Biophysica Acta·A J Miller, J J Zhou
Feb 24, 2001·The Plant Journal : for Cell and Molecular Biology·S MerlotJ Giraudat
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·A SzyrokiR Hedrich
May 30, 2002·The Plant Cell·Isabelle ChérelJean-Baptiste Thibaud
Nov 29, 2002·The EMBO Journal·Peter MichaelyRichard G W Anderson
Mar 29, 2003·Journal of Molecular Evolution·Guillaume PilotHervé Sentenac
Apr 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eric HosyHervé Sentenac
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
May 8, 2004·Trends in Plant Science·Alois SchweighoferIrute Meskiene
Apr 13, 2005·The Journal of General Physiology·Natalya IvashikinaRainer Hedrich
Nov 22, 2005·The Plant Journal : for Cell and Molecular Biology·Erwan MichardJean-Baptiste Thibaud
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Ingela JohanssonIngo Dreyer
Sep 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sung Chul LeeSheng Luan
Mar 28, 2008·The Plant Journal : for Cell and Molecular Biology·Florina VladSylvain Merlot
Mar 28, 2008·The Journal of Biological Chemistry·Kimberley B CravenWilliam N Zagotta
Mar 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anne LebaudyHervé Sentenac
Feb 3, 2009·Journal of Experimental Botany·Caroline SirichandraJeffrey Leung
Jun 23, 2009·Trends in Plant Science·Ingo Dreyer, Michael R Blatt
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Taishi UmezawaKazuo Shinozaki
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dietmar GeigerRainer Hedrich
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sung Chul LeeSheng Luan

❮ Previous
Next ❯

Citations

May 26, 2017·FEBS Letters·Claire Corratgé-FaillieTou Cheu Xiong
Apr 28, 2018·Journal of Experimental Botany·K R KonradR Hedrich
Feb 10, 2019·International Journal of Molecular Sciences·Isabelle Chérel, Isabelle Gaillard
Dec 18, 2018·FEBS Letters·Rainer WaadtKarin Schumacher
Jul 18, 2019·Plant, Cell & Environment·Govinal Badiger BhaskaraPaul E Verslues
Jun 17, 2020·The FEBS Journal·Malathi BheriGirdhar K Pandey
Apr 6, 2019·Frontiers in Plant Science·Paula RagelJosé M Pardo
May 6, 2019·International Journal of Molecular Sciences·Antonella LocascioLynne Yenush
Jan 28, 2020·Journal of Experimental Botany·Kun-Hsiang LiuJen Sheen
Jan 19, 2021·Plant, Cell & Environment·Kenji KomatsuYoichi Sakata
Dec 14, 2019·The Plant Pathology Journal·Chae Woo LimSung Chul Lee
Jan 12, 2021·Annual Review of Plant Biology·Omar Pantoja

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.