The Arabidopsis DNA mismatch repair gene PMS1 restricts somatic recombination between homeologous sequences.

Plant Molecular Biology
Liangliang LiFrançois J Belzile

Abstract

The eukaryotic DNA mismatch repair (MMR) system contributes to maintaining the fidelity of genetic information by correcting replication errors and preventing illegitimate recombination events. This study aimed to examine the function(s) of the Arabidopsis thaliana PMS1 gene (AtPMS1), one of three homologs of the bacterial MutL gene in plants. Two independent mutant alleles (Atpms1-1 and Atpms1-2) were obtained and one of these (Atpms1-1) was studied in detail. The mutant exhibited a reduction in seed set and a bias against the transmission of the mutant allele. Somatic recombination, both homologous and homeologous, was examined using a set of reporter constructs. Homologous recombination remained unchanged in the mutant while homeologous recombination was between 1.7- and 4.8-fold higher than in the wild type. This increase in homeologous recombination frequency was not correlated with the degree of sequence divergence. In RNAi lines, a range of increases in homeologous recombination were observed with two lines showing a 3.3-fold and a 3.6-fold increase. These results indicate that the AtPMS1 gene contributes to an antirecombination activity aimed at restricting recombination between diverged sequences.

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Citations

Aug 6, 2010·Molecular Biotechnology·Rodrigo L GomezClaudia P Spampinato
Jun 13, 2012·Journal of Biomedicine & Biotechnology·Claudia P Spampinato, Diego F Gomez-Casati
Aug 27, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sheh May TamRoger T Chetelat
Mar 4, 2011·The New Phytologist·Kim OsmanF Chris H Franklin
Dec 4, 2014·Journal of Experimental Botany·Luciana Daniela LarioClaudia Patricia Spampinato
Feb 23, 2017·Annual Review of Plant Biology·José M Gualberto, Kathleen J Newton
Jun 24, 2010·Molecular Biology Reports·Celina GallesClaudia P Spampinato
Nov 28, 2012·Molecular Biology Reports·Celina Galles, Claudia P Spampinato
Mar 21, 2020·Frontiers in Plant Science·Meng JiangHairui Cui
Feb 7, 2019·Frontiers in Plant Science·Elena Rakosy-TicanAdriana Aurori
Oct 8, 2020·Frontiers in Genetics·Prabha VermaVineet Gaur

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