The Arabidopsis FLC protein interacts directly in vivo with SOC1 and FT chromatin and is part of a high-molecular-weight protein complex

The Plant Journal : for Cell and Molecular Biology
Chris A HelliwellElizabeth S Dennis

Abstract

The Arabidopsis Flowering Locus C (FLC) protein is a repressor of flowering regulated by genes in the autonomous and vernalization pathways. Previous genetic and transgenic data have suggested that FLC acts by repressing expression of the floral integrator genes SOC1 and FT. We have taken an in vivo approach to determine whether the FLC protein interacts directly with potential DNA targets. Using chromatin immunoprecipitation, we have shown that FLC binds to a region of the first intron of FT that contains a putative CArG box, and have confirmed that FLC binds to a CArG box in the promoter of the SOC1 gene. MADS box proteins are thought to bind their DNA targets as dimers or higher-order multimers. We have shown that FLC is a component of a multimeric protein complex in vivo and that more than one FLC polypeptides can be present in the complex.

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Citations

Oct 3, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Silke StrackeHans-Peter Piepho
Sep 8, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Harsh RamanElizabeth Dennis
Jun 12, 2012·Molecular Genetics and Genomics : MGG·Amadou Oury DialloFathey Sarhan
Apr 18, 2013·Molecular Genetics and Genomics : MGG·Rebecca NitcherJorge Dubcovsky
Jun 25, 2013·Trends in Plant Science·Young Hun SongTakato Imaizumi
Aug 18, 2012·Nature Reviews. Genetics·Fernando Andrés, George Coupland
Feb 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Candice C SheldonW James Peacock
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandra N OliverBen Trevaskis
Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dong-Hwan Kim, Sibum Sung
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Weiwei DengElizabeth S Dennis
May 6, 2009·Journal of Experimental Botany·Ulf Lagercrantz
Apr 24, 2010·Journal of Experimental Botany·Jungeun Lee, Ilha Lee
Jan 28, 2012·Journal of Experimental Botany·Shiling JiangRobert D Hill
Dec 25, 2007·Plant & Cell Physiology·Carol R AnderssonElizabeth S Dennis
Nov 8, 2008·Plant & Cell Physiology·Masumi RobertsonElizabeth S Dennis
May 3, 2011·Plant & Cell Physiology·Tomohiro ImamuraHideyuki Takahashi
Feb 27, 2007·Genes & Development·Jeong Hwan LeeJi Hoon Ahn
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Feb 27, 2009·Genome Biology·Richard G H ImminkGerco C Angenent
Sep 8, 2011·Bioscience, Biotechnology, and Biochemistry·Hiroyoshi KuboMasayuki Nozue

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