The Arabidopsis GORK K+ -channel is phosphorylated by calcium-dependent protein kinase 21 (CPK21), which in turn is activated by 14-3-3 proteins

Plant Physiology and Biochemistry : PPB
P J M van KleeffA H de Boer

Abstract

Potassium (K+) is a vital ion for many processes in the plant and fine-tuned ion channels control the K+-fluxes across the plasma membrane. GORK is an outward-rectifying K+-channel with important functions in stomatal closure and in root K+-homeostasis. In this study, post-translational modification of the Arabidopsis GORK ion channel and its regulation by 14-3-3 proteins was investigated. To investigate the possible interaction between GORK and 14-3-3s an in vivo pull-down from an Arabidopsis protein extract with recombinant GORK C-terminus (GORK-C) indeed identified endogenous 14-3-3s (LAMBDA, CHI, NU) as binding partners in a phosphorylation dependent manner. However, a direct interaction between 14-3-3's and GORK-C could not be demonstrated. Since the pull-down of 14-3-3s was phosphorylation dependent, we determined GORK-C as substrate for CPK21 phosphorylation and identified three CPK21 phospho-sites in the GORK protein (T344, S518 and S649). Moreover, interaction of 14-3-3 to CPK21 strongly stimulates its kinase activity; an effect that can result in increased GORK phosphorylation and change in activity. Using the non-invasive vibrating probe technique, we measured the predominantly GORK mediated salt induced K+-efflux fr...Continue Reading

Citations

Apr 28, 2018·Journal of Experimental Botany·K R KonradR Hedrich
Feb 10, 2019·International Journal of Molecular Sciences·Isabelle Chérel, Isabelle Gaillard
Aug 2, 2019·The New Phytologist·Tiffany Yip Delormel, Marie Boudsocq
Dec 19, 2019·Journal of Integrative Plant Biology·Kong ChenYang Zhao
Apr 6, 2019·Frontiers in Plant Science·Paula RagelJosé M Pardo
Mar 3, 2020·Frontiers in Plant Science·Shunya Saito, Nobuyuki Uozumi
Apr 1, 2020·Frontiers in Plant Science·Lorella NavazioIldikò Szabò
Sep 26, 2019·Frontiers in Plant Science·Limin WangJulia M Davies
Jan 6, 2021·Journal of Integrative Plant Biology·Xuexue ChenZhizhong Gong
Jun 29, 2021·Frontiers in Plant Science·Maike Cosse, Thorsten Seidel

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