The Arabidopsis GPI-Anchored LTPg5 Encoded by At3g22600 Has a Role in Resistance against a Diverse Range of Pathogens

International Journal of Molecular Sciences
Muhammad Amjad AliHolger Bohlmann

Abstract

Arabidopsis contains 34 genes for glycosylphosphatidylinositol (GPI)-anchored LTPg proteins. A motif analysis has placed these into four groups. With one exception, all are produced with a signal peptide and are most likely attached to the cell membrane via the GPI anchor. Several of the LTPg genes across the four groups are downregulated in syncytia induced by the beet cyst nematode Heterodera schachtii. We have here studied At3g22600 encoding LTPg5, which is the most strongly downregulated LTPg gene. It is mainly expressed in roots, and a promoter::GUS line was used to confirm the downregulation in syncytia and also showed downregulation in galls of the root knot nematode Meloidogyne incognita. In contrast, infection with bacteria (Pseudomonas syringae) and fungi (Botrytis cinerea) led to the induction of the gene in leaves. This diverse regulation of LTPg5 indicated a role in resistance, which we confirmed with overexpression lines and a T-DNA mutant. The overexpression lines were more resistant to both nematode species and to P. syringae and B. cinerea, while a knock-out mutant was more susceptible to H. schachtii and P. syringae. Thus, LTPg5 encoded by At3g22600 is part of the Arabidopsis resistance mechanism against patho...Continue Reading

References

Jul 11, 1978·Molecular & General Genetics : MGG·M HolstersJ Schell
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 16, 2002·The Journal of Heredity·R E Voorrips
Dec 7, 2002·The Plant Cell·Allen SessionsStephen A Goff
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Shimon GepsteinMichal Bassani
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Jul 2, 2005·The EMBO Journal·Erik AndreassonJohn Mundy
Dec 30, 2006·The Plant Cell·Dinah QutobThorsten Nürnberger
Sep 25, 2008·BMC Bioinformatics·Andrea PierleoniRita Casadio
Nov 5, 2008·The Plant Journal : for Cell and Molecular Biology·Dagmar SzakasitsHolger Bohlmann
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jan 22, 2010·Current Protein & Peptide Science·E A KidoA M Benko-Iseppon
Apr 14, 2011·Molecular Plant·Monika M EdstamJohan Edqvist
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Mar 14, 2013·The Plant Journal : for Cell and Molecular Biology·Muhammad Amjad AliHolger Bohlmann
Dec 17, 2014·Bioinformatics·Bo HuGe Gao
Feb 24, 2015·Plant Physiology and Biochemistry : PPB·Hela SafiFaïçal Brini
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
May 3, 2018·Molecular Plant Pathology·Stacey N BarnesThomas J Baum
Sep 23, 2018·The Plant Journal : for Cell and Molecular Biology·Saet B Lee, Mi-Chung Suh

❮ Previous
Next ❯

Citations

Apr 28, 2021·Plant Physiology and Biochemistry : PPB·Huai-Na GaoYuan-Yuan Li

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transgenic
Assay

Software Mentioned

Genevestigator
ClustalW
SDS
MapChart
Gene Structure Display Server
MEGA
ExPASy
SignalP
MEME SUITE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.