The Arabidopsis J-protein AtDjB1 facilitates thermotolerance by protecting cells against heat-induced oxidative damage.

The New Phytologist
Wei ZhouBing Li

Abstract

AtDjB1 belongs to the J-protein family in Arabidopsis thaliana. Its biological functions in plants are largely unknown. In this study, we examined the roles of AtDjB1 in resisting heat and oxidative stresses in A. thaliana using reverse genetic analysis. AtDjB1 knockout plants (atj1-1) were more sensitive to heat stress than wildtype plants, and displayed decreased concentrations of ascorbate (ASC), and increased concentrations of hydrogen peroxide (H(2)O(2)) and oxidative products after heat shock. Application of H(2)O(2) accelerated cell death and decreased seedling viability in atj1-1. Exogenous ASC conferred much greater thermotolerance in atj1-1 than in wildtype plants, suggesting that a lower concentration of ASC in atj1-1 could be responsible for the increased concentration of H(2)O(2) and decreased thermotolerance. Furthermore, AtDjB1 was found to localize to mitochondria, directly interact with a mitochondrial heat-shock protein 70 (mtHSC70-1), and stimulate ATPase activity of mtHSC70-1. AtDjB1 knockout led to the accumulation of cellular ATP and decreased seedling respiration, indicating that AtDjB1 modulated the ASC concentration probably through affecting the function of mitochondria. Taken together, these results s...Continue Reading

References

Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·L Breeden, K Nasmyth
Jan 1, 1980·Molecular & General Genetics : MGG·C P GeorgopoulosM Feiss
Jan 15, 1994·European Journal of Biochemistry·J Becker, E A Craig
Jun 1, 1996·Plant Molecular Biology·B KroczynskaJ A Miernyk
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J C SedbrookP H Masson
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Ian M Moller
Aug 17, 2002·Current Opinion in Plant Biology·Steven NeillJohn Hancock
Sep 7, 2002·The Plant Cell·Cory A ChristensenGary N Drews
Jun 18, 2004·The Plant Journal : for Cell and Molecular Biology·Steven VandenabeeleFrank Van Breusegem
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Oct 7, 2004·Trends in Plant Science·Ron MittlerFrank Van Breusegem
Mar 17, 2005·Cellular and Molecular Life Sciences : CMLS·M P Mayer, B Bukau
Jul 1, 2005·Protein Science : a Publication of the Protein Society·Fritha HennessyGregory L Blatch
Apr 25, 2006·Reviews of Physiology, Biochemistry and Pharmacology·E A CraigA Andrew
Oct 25, 2006·The Plant Journal : for Cell and Molecular Biology·Ayako NishizawaShigeru Shigeoka
May 8, 2007·Current Opinion in Plant Biology·Sachin KotakKlaus-Dieter Scharf
Jan 19, 2008·The Journal of Biological Chemistry·Nobuhiro SuzukiRon Mittler
Aug 23, 2008·Journal of Proteomics·Anna Maria TimperioLello Zolla

❮ Previous
Next ❯

Citations

Nov 20, 2012·Cell Stress & Chaperones·Neelam K SarkarAnil Grover
Dec 25, 2012·Cell Stress & Chaperones·Neelam K SarkarAnil Grover
Nov 15, 2013·Journal of Experimental Botany·Fanying KongQingwei Meng
Aug 17, 2014·Plant Physiology and Biochemistry : PPB·Zongliang XiaJianjun Liu
Aug 28, 2012·Plant Science : an International Journal of Experimental Plant Biology·Ching-Hui YehYee-Yung Charng
Jun 3, 2015·Annals of Botany·Maria Concetta de PintoLaura De Gara
Mar 25, 2015·Journal of Experimental Botany·Guodong WangQingwei Meng
Dec 2, 2014·Plant Physiology and Biochemistry : PPB·Dhruv LavaniaAnil Grover
Apr 6, 2018·Genome Génome / Conseil National De Recherches Canada·Bin ZhangDong-Hong Chen
Oct 24, 2013·Journal of Integrative Plant Biology·Fanying KongQingwei Meng
Jan 19, 2019·International Journal of Molecular Sciences·Guodong WangQingwei Meng
Dec 5, 2020·International Journal of Biological Macromolecules·Hongjie FengHeqin Zhu
Jan 1, 2019·Critical Reviews in Plant Sciences·Amit K VermaChandan Sahi
Aug 4, 2021·Journal of Experimental Botany·Shin-Hee HanChung-Mo Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.